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[目的]观察青黛颗粒对溃疡性结肠炎(UC)实验大鼠血清白细胞介素6(IL-6)和白细胞介素10(IL-10)水平的影响,探讨其治疗UC可能的作用机制。[方法]将52只SD大鼠采用三硝基苯磺酸(TNBS)法制备UC大鼠模型。造模结束后第2天取4只大鼠处死确定造模成功,余48只随机均分为6组:正常组,模型组,柳氮磺胺嘧啶(SASP)阳性药物治疗(SASP)组,青黛颗粒低、中、高剂量组,各8只。造模后第3天开始各组动物灌胃给药,连续给药10 d,实验第14天通过腹主动脉采血取血清,用ELISA法测定血清IL-6、IL-10水平。[结果]青黛颗粒低、中、高3个剂量组与模型组比较,血清IL-6水平显著降低,IL-10水平升高(P<0.01);青黛颗粒高剂量组血清IL-6I、L-10水平变化与SASP组比较,差异无统计学意义(P>0.05)。[结论]青黛颗粒治疗实验性UC的作用机制可能与降低IL-6水平,升高IL-10水平有关。
[Objective] To observe the effect of indigo granule on the levels of serum interleukin 6 (IL-6) and interleukin 10 (IL-10) in rats with ulcerative colitis (UC) and to explore its possible mechanism of action in treating UC. [Method] 52 SD rats were used to prepare UC rat model by trinitrobenzene sulfonic acid (TNBS) method. Four rats were sacrificed on the second day after the model was established, and the remaining 48 animals were randomly divided into 6 groups: normal group, model group, SASP-positive drug treatment group (SASP) In the low, medium and high dose groups, 8 mice each. On the third day after the model was established, the animals in each group were given gavage for 10 days. Serum was collected from the abdominal aorta on the 14th day of experiment and the levels of IL-6 and IL-10 were measured by ELISA. [Result] Compared with the model group, the serum levels of IL-6 and the level of IL-10 increased (P <0.01) in the three dosage groups of low, middle and high Qingdai granules. Compared with model group, -10 levels compared with the SASP group, the difference was not statistically significant (P> 0.05). [Conclusion] The mechanism of Qing Dai Granule in treating experimental UC may be related to decreasing IL-6 level and increasing IL-10 level.