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目的：进一步明确肺癌在 8p21～ 23区域等位基因杂合性丢失（ loss of heterozygosity, LOH）的频率与共同缺失区范围，以便分离该区域内与肺癌相关的候选抑瘤基因。方法：应用位于 8p21～ 23的 16个微卫星多态标记，对 32例肺癌组织和 2例肺癌细胞系进行 LOH分析。结果： 32例肺癌组织与 2例肺癌细胞系中共 20例（ 58.82％）存在至少一个位点的杂合性丢失，缺失频率最高的位点是 8p21.1的 NEFL与 D8s1771及 8p21.3的 D8s133，其频率分别为 45.16％（ 14/31）、 37.03％（ 11/27）和 35.72％（ 10/28）。 7例患者在 D8s1771～ D8s1477间存在连续性共同缺失。结论：肺癌在 8p21～ 23的最小共同缺失区位于 8p21的 D8s1771～ D8s1477之间，该区域可能存在一个与肺癌发生发展密切相关的抑瘤基因。
OBJECTIVE: To further clarify the frequency and common deletion region of loss of heterozygosity (LOH) in the 8p21 ~ 23 region of lung cancer in order to isolate the candidate tumor suppressor genes associated with lung cancer in this region. Methods: LOH analysis was performed on 32 lung cancer tissues and 2 lung cancer cell lines using 16 microsatellite polymorphism markers located at 8p21 ~ 23. RESULTS: Totally 20 (58.82%) of 32 lung cancer tissues and 2 lung cancer cell lines had at least one loss of heterozygosity. The most frequent deletion sites were NEFL and D8s1771 of 8p21.1 and D8s133 of 8p21.3 With frequency of 45.16% (14/31), 37.03% (11/27) and 35.72% (10/28), respectively. Seven patients had a common deletion between D8s1771 ~ D8s1477. Conclusion: The smallest common deletion region of 8p21-23 in lung cancer is located between D8s1771 ~ D8s1477 of 8p21. There may be a tumor suppressor gene closely related to the development of lung cancer in this region.