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对水稻(Oryza sativa L.)基因文库中分离到的U2snRNA基因FDRGU2.3进行序列分析,其编码区与小麦(Triticum aestivum L、)、玉米(Zea mays L.)、豌豆(Pisum sativum L.)及拟南芥(Arabidopsis thaliana(L.)Heyhy.)等植物U2基因的同源性均大于80％,且5＇端70个碱基高度保守。在基因编码区上游-70及-30区分别包含有植物UsnRNA基因特有的上游顺序元件(USE)及类TATA元件。同其它植物一样,水稻U2.3snRNA的二级结构也有保守的4个茎环区。其中环Ⅱ的结构与单子叶植物中的小麦和玉米相同,但与双子叶植物的豌豆和拟南芥存在明显差异。环Ⅳ的结构在单子叶和双子叶植物中亦有不同的变化。这些差异可能意味着单子叶和双子叶植物的剪接机构有所区别。
The U2snRNA gene FDRGU2.3 isolated from rice (Oryza sativa L.) gene library was sequenced and its coding region was compared with that of wheat (Triticum aestivum L), maize (Zea mays L.), pea (Pisum sativum L.) And Arabidopsis thaliana (L.) Heyhy.) Are more than 80% homologous to the U2 gene in plants, and the 70 bases at the 5 ’end are highly conserved. The upstream sequence elements (USE) and TATA-like elements of the plant UsnRNA gene are contained in the -70 and -30 regions upstream of the gene coding region, respectively. Like other plants, the secondary structure of rice U2.3snRNA also has four conserved stem-loop regions. The structure of ring II is the same as wheat and corn in monocotyledons, but there are obvious differences between dicotyledonous peas and Arabidopsis. The structure of ring IV varies between monocotyledonous and dicotyledonous plants. These differences may imply a difference between the splice machinery of monocotyledonous and dicotyledonous plants.