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利用 MTT快速比色法 ,以长春新碱 (Vincristin Sulfas)为阳性对照 ,研究了从玄参科(Scrophulariaceae)兰石草属兰石草 (L ancea tibetica Hook Fet Thoms)中提取的呋喃骈呋喃木脂素对人肝癌细胞 (SMMC- 772 1) ,人宫颈癌细胞 (Hela) ,中国仓鼠肺成纤维细胞 (V79)及小鼠黑色素肉瘤细胞 (B16 )的体外抗肿瘤活性 .结果显示 :呋喃骈呋喃木脂素 L C1对 4种细胞均有较强的抑制作用 ,IC50 在 4 0 .2～ 87.9μg/ m L范围 ,它对 B16的抑制作用强于长春新碱 .但是 ,L C2和 L C3均无抗癌活性 (>4 0 0 μg/ m L) ,可能 L C2和 L C3的酚羟基上的 H被糖基取代而使抗癌活性丧失 .
The furanofuran wood extracted from L ancea tibetica Hook Fet Thoms from Scrophulariaceae was studied using MTT rapid colorimetry and Vincristin Sulfas as a positive control. Antitumor activity of lipoproteins on human hepatoma cells (SMMC-772 1), human cervical cancer cells (Hela), Chinese hamster lung fibroblasts (V79) and mouse melanoma sarcoma cells (B16). The results showed that: Furanol L C1 has a strong inhibitory effect on all four kinds of cells. The IC50 is in the range of 40. 2-87.9 μg/m L. It has stronger inhibitory effect on B16 than vincristine. However, L C2 and L C3 had no anti-cancer activity (>400 μg/m L). It may be that H on the phenolic hydroxyl group of L C2 and L C3 was replaced by glycosyl and the anti-cancer activity was lost.