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目的探讨14-3-3β蛋白对胶质瘤细胞系生物学行为的影响。方法应用分子生物学技术构建14-3-3β蛋白的真核表达质粒pc DNA 3.1(-)-14-3-3β及干扰质粒pc DNATM6.2-GW/Em GFPmiR-14-3-3β,应用MTT、集落形成、Transwell侵袭试验研究14-3-3β蛋白对人胶质瘤细胞株U251增殖及侵袭等生物学行为的影响。结果过表达14-3-3β能够明显促进U251细胞(人胶质瘤系)的侵袭和增殖过程;而应用干扰质粒抑制14-3-3β表达后,U251细胞的侵袭和增殖能力则受到显著抑制。结论 14-3-3β对于U251细胞(人胶质瘤系)蛋白的侵袭和增殖能力起到明确的促进作用,其在人类胶质瘤的发生和发展过程中可能也起到促癌作用。
Objective To investigate the effect of 14-3-3β on the biological behavior of glioma cell lines. Methods The eukaryotic expression plasmid pcDNA 3.1 (-) - 14-3-3β of 14-3-3βprotein was constructed by using molecular biology techniques and the interference plasmid pcDNA-DNATM6.2-GW / Em GFPmiR-14-3-3β was constructed. The effects of 14-3-3βprotein on the biological behaviors of human glioma cell line U251 such as proliferation and invasion were investigated by MTT, colony formation and Transwell invasion assay. Results The overexpression of 14-3-3β significantly promoted the invasion and proliferation of U251 cells (human glioma cell line). However, the invasion and proliferation of U251 cells were significantly inhibited by interfering with the expression of 14-3-3β . Conclusion 14-3-3β may promote the invasion and proliferation of U251 cells (human glioma), which may also play a role in carcinogenesis and progression of human glioma.