The study was intended to discuss the appropriate application concentrations of Mg, Zn and Mo fertilizers for high yield and good quality of leaf-use ginkgo. Effects of spraying different levels of Mg, Zn and Mo fertilizers on leaf yield and medicinal quality of two-year-old ginkgo seedlings were tested by indoor stimulation experiment and pot experiment. The main results were submitted as follows: firstly, the combined application of Mg, Zn and Mo fertilizers increased leaf thickness, leaf area, single plant leaf area, and leaf biomass. The best treatment was Mo③ (2%Mg+0.3%Zn+0.1%Mo) which increased the leaf biomass by 64.9% compared with that of control. Secondly, the positive effect of combining Mg with Zn and Mo fertilizers on chlorophyll and soluble protein contents decreased with the increasing Mg concentration. The same was also the effects of combining Zn with Mg and Mo fertilizers as well as combining Mo with Mg and Zn fertilizers on chlorophyll and soluble sugar contents. In contrast, the effect on soluble protein content correlated positively with Zn or Mo concentration. Thirdly, the combined application of Mg, Zn and Mo fertilizers increased total flavonoid and terpene lactones contents, total flavonoid and terpene lactones yields per plant. The best treatment was Mo③ which increased total flavonoid yield per plant by 2.96 times and total terpene lactones yield per plant by 1.70 times compared with that of control. Fourthly, high-concentration fertilizers as Mg④, Zn④ and Mo④ decreased the positive effect of the combined application of Mg, Zn and Mo fertilizers on some indexes to different degrees. The results indicated that appropriate concentration of the three fertilizers could increase chlorophyll, soluble sugar, soluble protein contents and improve ginkgo growth and medicinal quality, as well as increase component factors of leaf yield and medicinal quality. In the experiment, Mo③ was the perfect combination in order to increase ginkgo leaf yield and medicinal composition content and yield. The study was intended to discuss the appropriate application of concentrations of Mg, Zn and Mo fertilizers for high yield and good quality of leaf-use ginkgo. Effects of spraying different levels of Mg, Zn and Mo fertilizers on leaf yield and medicinal quality of two- year-old ginkgo seedlings were tested by indoor stimulation experiment and pot experiment. The main results were submitted as follows: first, the combined application of Mg, Zn and Mo fertilizers increased leaf thickness, leaf area, single plant leaf area, and leaf biomass . The best treatment was Mo③ (2% Mg + 0.3% Zn + 0.1% Mo) which increased the leaf biomass by 64.9% compared with that of control. Secondly, the positive effect of combining Mg with Zn and Mo fertilizers on chlorophyll and soluble The same was also the effects of combining Zn with Mg and Mo fertilizers as well as combining Mo with Mg and Zn fertilizers on chlorophyll and soluble sugar contents. I nly contrast, the effect on soluble protein content positively with Zn or Mo concentration. Thirdly, the combined application of Mg, Zn and Mo fertilizers increased total flavonoid and terpene lactones contents, total flavonoid and terpene lactones yield per plant. The best treatment was Mo③ which increased total flavonoid yield per plant by 2.96 times and total terpene lactones yield per plant by 1.70 times compared with that of control. Fourthly, high-concentration fertilizers as Mg④, Zn④ and Mo④ decreased the positive effect of the combined application of Mg, Zn and Mo fertilizers on some indexes to different degrees. The results indicates that appropriate concentration of the three fertilizers could increase chlorophyll, soluble sugar, soluble protein contents and improve ginkgo growth and medicinal quality, as well as increase component factors of leaf yield and medicinal quality. In the experiment, Mo③ was the perfect combination in order to increase ginkgo le af yield andmedicinal composition content and yield.