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目的：分析肉鸡养殖、屠宰和销售不同环节来源大肠杆菌对β‐内酰胺类药物的敏感性及CTX‐M 型超广谱β‐内酰胺酶（ESBLs）的携带状况，探讨大肠杆菌在肉鸡养殖、屠宰和销售环节上可能存在的耐药性传播。方法对从山东大型肉鸡养殖场、屠宰场分离的373株大肠杆菌进行药物敏感性检测、blaCTX‐M基因核酸检测及序列分析。结果菌株对头孢他啶、头孢噻肟和哌拉西林存在高水平的耐药，耐药率依次是89．3％、92．8％和91．7％，但对哌拉西林／他唑巴坦仍保持敏感。菌株blaCTX‐M基因的携带率高，达54．7％（204／373），来自养殖和销售等不同环节的菌株携带的blaCTX‐M基因集中在4个亚型，分别是 blaCTX‐M‐55、blaCTX‐M‐65、blaCTX‐M‐14和 blaCTX‐M‐27。结论鸡源大肠杆菌对青霉素及头孢菌素类药物有较高的耐药率，对酶抑制剂复方制剂仍保持较高的敏感率。携带blaCTX‐M基因的菌株来自养殖和销售等不同的环节，主要集中在4个亚型，提示肉鸡食品链不同环节可能存在大肠杆菌耐药性的传播。“,”Objective To analyse the antimicrobial resistances profiles andβ‐lactamases CTX subtypes of the Escherichia coli strains isolated from different poultry production process of breeding ,slaughtering and retails and ex‐plore the transmission of drug resistance of Escherichia coli in chicken breeding ,slaughter and sale .Methods Anti‐microbial resistant profiles and blaCTX‐M gene were tested in three hundred and seventy‐three Escherichia coli that from poultry production processGenotypes of CTX‐M type ESBLs were identified by PCR and DNA sequencing .Results Three hundred and seventy‐three strains were highly resistant to ceftazidime ,cefotaxime and piperacillin ,which re‐sistance rate attached to 89 .3% ,92 .8% and 91 .7% respectively .While they were still susceptible to piperacillin/tazobactam .The rate of the strains carrying the blaCTX‐M gene was up to 54 .7% (204/373) ,focused on four subtypes blaCTX‐M‐55 ,blaCTX‐M‐65 ,blaCTX‐M‐14 and blaCTX‐M‐27 .Conclusion E .coli strains isolated from poultry production were high resistant to cephalosporins and penicillin ,while still sensitive to β‐lctamase inhibitor compound .The strains carrying blaCTX‐M gene were mainly isolated from the breeding and retail process ,focused on four subtypes ,inferring that the possible blaCTX‐M gene transfer during the poultry food production .