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[目的]建立离子色谱法测定转基因大豆中草甘膦残留量的方法。[方法]采用沉淀法对大豆样品进行前处理,并用离子色谱法测定净化后大豆样品中草甘膦含量。[结果]采用亚铁氰化钾-乙酸锌沉淀法对大豆样品进行的蛋白质沉淀净化效果优于乙腈沉淀法。经逐级纯化,取净化液进行离子色谱分析。结果表明:样品加标回收率在85.2%~94.0%之间,最低检出限为0.015 mg/L,草甘膦标准曲线的线性相关系数为0.999。[结论]该方法净化处理效果好,准确度和精密度高,检出限低,适于转基因大豆中草甘膦的定性定量分析。
[Objective] The research aimed to establish a method for determining glyphosate residues in transgenic soybean by ion chromatography. [Method] The soybean sample was pretreated by precipitation method and the content of glyphosate in purified soybean sample was determined by ion chromatography. [Result] The protein precipitation purification effect of soybean sample by using potassium ferrocyanide - zinc acetate precipitation method was better than acetonitrile precipitation method. After gradual purification, take the purification solution for ion chromatography. The results showed that the recoveries of spiked samples ranged from 85.2% to 94.0% with the lowest detection limit of 0.015 mg / L and the linear correlation coefficient of glyphosate standard curve was 0.999. [Conclusion] The method has good purification effect, high accuracy and precision, and low detection limit. It is suitable for qualitative and quantitative analysis of glyphosate in transgenic soybean.