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针对中国大豆灰斑病 1号生理小种 ,以抗所有生理小种的品系东农 4 0 5 6 6为母本 ,以感 1号生理小种的品种东农 4 10为父本配制杂交组合 ,杂交得到F2 代后连续自交 3代得到F5代群体。该群体经人工接种灰斑病 1号生理小种后 ,利用BSA法对 5 0 0个SSR标记进行筛选 ,其中 3个标记Satt5 6 5、SOYGPATR和Satt396在抗、感池间表现出稳定的多态性 ,并且在F2 代个体中表现出抗性与多态性协同分离的趋势。这 3个标记与抗性基因的连锁顺序为Satt5 6 5 -SOYGPATR-Hrcs1-Satt396 ,它们与抗性基因的连锁距离分别为 12 .7cM、6 .5cM、14 .7cM。推测抗大豆灰斑病 1号生理小种的基因可能位于C1连锁群上。
Aiming to the Chinese races Gray Soybean (Sciurocephalia spp.) No.1 races, Dongnong 4 0 5 6 6, which is resistant to all races, was used as the female parent and Dongnong 4 10 After F2 generation, the plants were selfed for 3 generations to obtain F5 generation. After artificial inoculation of the gray spot # 1 race, the population was screened for 540 SSR markers by BSA method. Among them, three markers, Satt5 6 5, SOYGPATR and Satt396, showed a more stable And showed a trend of coordinated segregation of resistance and polymorphism in F2 individuals. The linkage of the three markers to the resistance gene was Satt565-SOYGPATR-Hrcs1-Satt396, and the linkage distances to the resistance genes were 12.7 cM, 6.5 cM and 14.7 cM, respectively. It is speculated that the gene of Physiological Resistance to Soybean Gray Leaf Spot No.1 may be located on the C1 linkage group.