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目的探讨当归挥发油对自发性高血压大鼠肝脏脂代谢的改善作用及其机理。方法将40只8周龄雄性自发性高血压大鼠随机分为模型组,当归挥发油低、中、高剂量组和缬沙坦组,并用同周龄的Wistar大鼠作为正常对照组。分别灌胃4周后,取大鼠肝脏组织,HE染色观察肝脏组织形态学的变化,免疫印迹法检测TRIB1、TRL3、AHSG蛋白水平的表达。结果 HE染色显示,与模型组比较,当归挥发油各组病变分别有不同程度的减轻,缬沙坦组未见明显异常;TRIB1、TRL3、AHSG蛋白在模型组,当归挥发油中、高剂量组表达上调,且呈差异性表达,在当归不同剂量组之间亦呈差异性上调表达。结论当归挥发油对自发性高血压大鼠脂代谢有调节作用,可能通过上调TRIB1、TRL3、AHSG等蛋白的表达量抑制动脉粥样硬化,改善自发性高血压大鼠的脂代谢而达到降压的目的。
Objective To investigate the effect of angelica volatile oil on hepatic lipid metabolism in spontaneously hypertensive rats and its mechanism. Methods Forty eight-week-old male spontaneously hypertensive rats were randomly divided into model group, angelica volatile oil low, medium and high dose groups and valsartan group. Wistar rats of the same age were used as normal control group. After 4 weeks, the liver tissues of rats were taken out and the changes of liver histomorphology were observed by HE staining. The expressions of TRIB1, TRL3 and AHSG were detected by Western blot. Results HE staining showed that compared with the model group, the lesions of Angelica sinensis volatile oil groups were relieved to varying degrees, and there was no obvious abnormality in the valsartan group. The expressions of TRIB1, TRL3 and AHSG were up-regulated in model group and Angelica sinensis volatile oil groups , And was differentially expressed in the different doses of Angelica also showed a difference between up-regulated expression. Conclusion Angelica Volatile Oil regulates lipid metabolism in spontaneously hypertensive rats. It may inhibit the atherosclerosis by increasing the expression of TRIB1, TRL3 and AHSG, and improve the lipid metabolism in spontaneously hypertensive rats. purpose.