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目的寻找可作为急性淋巴细胞白血病恶性克隆的基因标记。方法应用ＰＣＲ技术扩增Ｔ细胞受体γ链基因重排，选用四种限制性核酸内切酶消化扩增产物。结果初诊或复发１０例急性淋巴细胞白血病病人中，有５例出现清晰扩增带，约４００ｂｐ，扩增物经四种限制性核酸内切酶消化后形成的限制性核酸内切酶图谱各具特色；５例处于缓解期的急性淋巴细胞白血病病人，有４例被检出清晰扩增带。结论应用ＰＣＲ扩术扩增Ｔ细胞受体γ链基因重排，能用于检测急性淋巴细胞白血病微小残留病，如果结合限制性核酸内切酶图谱分析，则能用于研究白血病细胞的克隆演化
Objective To find genetic markers that can be used as malignant clones in acute lymphoblastic leukemia. Methods PCR was used to amplify the rearrangement of T cell receptor gamma chain gene. Four restriction endonucleases were used to digest the amplified products. Results Of the 10 patients with newly diagnosed or relapsed acute lymphoblastic leukemia, 5 showed clear bands of amplification, about 400 bp, and the restriction endonuclease map formed after amplification of the amplicons was digested by four restriction endonucleases. Features; 5 cases of acute lymphoblastic leukemia patients in remission, 4 cases were detected in clear bands. Conclusion PCR amplification can be used to amplify T cell receptor γ chain gene rearrangement. It can be used to detect minimal residual disease in acute lymphoblastic leukemia. If combined with restriction endonuclease mapping, it can be used to study the clonal evolution of leukemia cells.