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目的观察参麦注射液对体外培养外周血来源内皮祖细胞(endothelial progenitor cells,EPCs)数量及增殖、迁移、粘附功能的影响。方法密度梯度离心法获取人外周血单个核细胞,FITC-荆豆凝集素I(FITC-UEA-1)、DiL-乙酰化低密度脂蛋白(DiL-acLDL)荧光双染鉴定;单个核细胞培养4d后进行实验分组,分为对照组和参麦注射液治疗组,参麦注射液治疗组加入不同浓度参麦注射液(分别为0.01、0.1、0.15、0.3mg/L)培养48h,然后分别采用四氮唑溴盐比色法(MTT)、改良的Boyden小室和粘附能力测定来观察EPCs的增殖、迁移和粘附能力。结果不同浓度的参麦注射液对EPCs有不同作用,0.1、0.15mg/L主要表现为使EPCs功能增强,0.3mg/L时贴壁细胞数量减少及增殖、迁移、粘附功能减弱,0.01mg/L与未加药组比较无统计学意义。结论参麦注射液可影响培养EPCs的数量及其部分生物学功能。
Objective To observe the effect of Shenmai injection on the number and proliferation, migration and adhesion of endothelial progenitor cells (EPCs) in peripheral blood cultured in vitro. Methods Peripheral blood mononuclear cells were obtained by density gradient centrifugation. FITC-UEA-1 and DiL-acLDL were identified by fluorescence double staining; mononuclear cell culture. After 4 days, the experimental groups were divided into control group and Shenmai injection treatment group. Shenmai injection treatment group was added with different concentrations of Shenmai injection (0.01, 0.1, 0.15, 0.3 mg/L, respectively) for 48 hours, and then they were separately Measure the proliferation, migration, and adhesion of EPCs using the tetrazolium bromide colorimetric assay (MTT), modified Boyden chamber, and adhesion assay. Results Different concentrations of Shenmai injection had different effects on EPCs, 0.1 and 0.15 mg/L mainly showed enhanced EPCs function, and the number of adherent cells decreased and the proliferation, migration, and adhesion decreased at 0.01 mg/mL. There was no statistically significant difference between the /L and untreated groups. Conclusion Shenmai injection can affect the number of cultured EPCs and some of their biological functions.