论文部分内容阅读
目的:观察新血府逐瘀汤与血府逐瘀汤对AS大鼠主动脉病理形态学及免疫调节细胞因子HMGB-1、TGF-β表达的影响。方法:健康雄性Wistar大鼠40只,随机分为对照组、模型组、立普妥组、血府逐瘀汤组及新血府逐瘀汤组,每组8只。除空白组,均采用高脂饲料联合维生素D3方法复制AS模型。各治疗组第3周灌胃给药,新血府逐瘀汤组18 g/(kg·d),血府逐瘀汤组15 g/(kg·d)灌胃,西药组予立普妥2 mg/(kg·d)灌胃;对照组及模型组则分别灌服等容积生理盐水,连续8周;HE染色镜下观察胸主动脉病理学改变,免疫组织化学法检测胸主动脉HMGB-1、TGF-β表达。结果:HE染色显示:新血府逐瘀汤组与血府逐瘀汤组相比,内膜厚度有统计学差异(P<0.05);免疫组化评分显示:与模型组相比,新血府逐瘀汤组HMGB-1明显降低、TGF-β明显升高,具有显著统计学意义(P<0.01);与血府逐瘀汤组相比,新血府逐瘀汤组降低HMGB-1、升高TGF-β表达均有统计学差异(P<0.05)。结论:新血府逐瘀汤在降低内膜厚度、调节免疫细胞因子表达方面优于传统血府逐瘀汤。
OBJECTIVE: To observe the effects of Xuefuzhuyu Decoction and Xuefu Zhuyu Decoction on the pathological changes of the aorta and the expressions of immunoregulatory cytokines HMGB-1 and TGF-β in AS rats. Methods: Forty healthy male Wistar rats were randomly divided into control group, model group, Lipitor group, Xuefuzhuyu Decoction group and Xinxuefuzhuyu Decoction group, with 8 rats in each group. In addition to the blank group, high-fat diet combined with vitamin D3 copy AS model. Each treatment group was intragastrically administrated at the third week, and Xinhua Fuzhuyu Decoction group (18 g / (kg · d)) and Xuefu Zhuyu Decoction group (15 g / (kg · d) 2 mg / (kg · d) intragastrically. The control group and model group were given the same volume of normal saline for 8 weeks respectively. Pathological changes of the thoracic aorta were observed by HE staining. -1, TGF-β expression. Results: HE staining showed that the thickness of intima of Xuefuzhuyu Decoction group was significantly different from that of Xuefu Zhuyu Decoction group (P <0.05), and the immunohistochemical score showed that compared with model group, Compared with Xuefuzhuyu Decoction group, Xinxuefuzhuyu Decoction group decreased HMGB-1, HMGB-1 significantly decreased, TGF-β significantly increased (P <0.01) , Increased TGF-β expression were statistically significant (P <0.05). Conclusion: Xin Xue Fu Zhu Yu Decoction is superior to traditional Xuefu Zhuyu Decoction in reducing the intima thickness and regulating the expression of immune cytokines.