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我们从1985年开始研究毛白杨的扦插生根问题,采用系列浓度的各种生长素类化合物处理长度为8—10厘米的硬枝插穗,在恒温培养箱中观察愈伤组织形成和生根情况。试验发现毛白杨愈伤组织生根比较困难,单纯增加生长素浓度虽能诱导出发达的愈伤组织,但对加速不定根的产生并没有多大帮助。前人在调节生长素与细胞分裂素比例控制烟草髓组织培养的器官分化的经典试验早已证明,虽然生长素比例较大时有利于生根,但在细胞分裂素水平过低或完金缺乏时,只增加生长素所形成的愈伤
We started from 1985 to study the cutting rooting of Populus tomentosa, a series of concentrations of various auxin compounds for processing length of 8-10 cm hardwood cuttings, observed in the incubator callus formation and rooting. It was found that the rooting of Populus tomentosa callus was more difficult. Simply adding auxin concentration could induce the developed callus, but it did not help to accelerate adventitious root formation. Previous studies on the regulation of organ differentiation in tobacco pulp tissue by regulating the ratio of auxin and cytokinin have long proved that although the ratio of auxin is favorable for rooting, when the level of cytokinin is too low or the deficiency of gold is complete, Only increase the callus formed by auxin