论文部分内容阅读
背景:运动可提高胰岛素的敏感性和作用效果,其对胰岛素的影响与骨骼肌细胞胰岛素受体底物2的介导功能密切相关。目的:了解不同强度运动对大鼠骨骼肌细胞胰岛素受体底物2蛋白含量及磷酸化程度的影响。设计、时间及地点:随机对照动物实验,于2008-04/06在辽宁师范大学体育学院运动人体科学实验室完成。材料:将60只健康SD大鼠随机分为正常对照组、糖尿病模型对照组、糖尿病模型运动强度15,20,25,30m/min组,每组10只。方法:以腹腔内注射链脲左霉素制备糖尿病大鼠模型,正常对照组注射等量的柠檬酸缓冲液。采用15,20,25,30m/min运动强度对糖尿病大鼠进行干预,1次/d,50min/次,每周训练6d,共6周。正常对照组及糖尿病模型对照组不进行运动干预。主要观察指标:以Westernblot免疫印迹法检测各组大鼠骨骼肌细胞胰岛素受体底物2蛋白含量及磷酸化程度。结果:与糖尿病模型对照组相比,15,20,25,30m/min不同强度运动组大鼠股四头肌胰岛素受体底物2蛋白含量分别升高17%、24.4%、23.4%和21.3%(P<0.05,P<0.01),股四头肌胰岛素受体底物2磷酸化水平分别升高34.7%、37.2%、40.2%和38.8%(P<0.01),但各组仍没有恢复到正常对照组水平。不同运动强度组间大鼠骨骼肌细胞胰岛素受体底物2的表达和磷酸化程度无统计学差异。结论:在不同运动强度影响下,糖尿病大鼠骨四头肌胰岛素受体底物2含量均增加,但增加的幅度存在差异,以20m/min及以上的强度运动效果较好,股四头肌胰岛素受体底物2的磷酸化程度变化不明显。
BACKGROUND: Exercise improves insulin sensitivity and effect, and its effect on insulin is closely related to the mediation function of insulin receptor substrate 2 in skeletal muscle cells. Objective: To investigate the effect of different intensity exercise on insulin receptor substrate 2 protein content and phosphorylation in rat skeletal muscle cells. DESIGN, TIME AND SETTING: A randomized controlled animal experiment was performed at Sports Human Science Laboratory of Liaoning Normal University from April to June 2008. MATERIALS: Sixty healthy SD rats were randomly divided into normal control group, diabetic model control group and diabetic model with exercise intensity of 15, 20, 25 and 30 m / min, with 10 rats in each group. Methods: Diabetic rats were induced by intraperitoneal injection of streptozotocin. Normal control group was injected with the same amount of citrate buffer solution. Diabetic rats were treated with 15, 20, 25 and 30m / min exercise intensity for 1 week, 50 minutes and 6 weeks per week for 6 weeks. Normal control group and diabetic model control group did not exercise intervention. MAIN OUTCOME MEASURES: Protein content and phosphorylation of insulin receptor substrate 2 in skeletal muscle cells of rats in each group were detected by Western blot. RESULTS: Compared with the diabetic model control group, the protein content of insulin receptor substrate 2 in the quadriceps femoris increased by 17%, 24.4%, 23.4% and 21.3% respectively at 15,20,25,30 m / min (P <0.05, P <0.01). The phosphorylation of insulin receptor substrate 2 in the quadriceps femoris increased by 34.7%, 37.2%, 40.2% and 38.8% (P <0.01) To normal control group level. There was no significant difference in the expression and phosphorylation of insulin receptor substrate 2 in skeletal muscle cells between different exercise intensity groups. CONCLUSION: Under the influence of different exercise intensity, the content of insulin receptor substrate 2 in the biceps femoris muscle of diabetic rats increased, but the amplitude of increase was different. The exercise intensity of 20m / min and above was good. The quadriceps femoris Insulin receptor substrate 2 changes in the degree of phosphorylation is not obvious.