【摘 要】
：
Background Control of hypersecretion of certain hormones is one of the key targets in the treatment of pituitary adenomas. RNA interference has been shown to in
【机 构】
：
Department of Neurosurgery,State Key Laboratory of Molecular Oncology
论文部分内容阅读
Background Control of hypersecretion of certain hormones is one of the key targets in the treatment of pituitary adenomas. RNA interference has been shown to inhibit protein expression, and thus it may represent a promising method for the treatment of pituitary adenomas. In the present study, transfection efficiency of small interfering RNA (siRNA) was optimized in human prolactinoma cells.Methods First, a method was optimized to extract highly purified human prolactinoma cells in vitro. The extracted cells were verified to retain the physiological features of prolactin (PRL) secretion. Second, three conditions for siRNA transfection were tested by the evaluation of transfection efficiency and cell viability. The proper transfection condition was verified for human prolactinoma cells. Third, the siRNA for prolactin was transfected into the human prolactinoma cells, and the suppression of PRL mRNA was evaluated by quantitative real-time reverse transcription-PCR.Conclusion It is possible to inhibit hormone hypersecretion by RNA interference, that may eventually enable therapeutic siRNA drugs developed.
其他文献
Background There is currently no consensus regarding the best graft type for anterior cruciate ligament reconstruction.Therefore,the aim of this study was to in
在当前利用实测光谱数据通过线性回归方法构建水体叶绿素a浓度反演模型的过程中,通常的数据处理流程是首先通过相关分析确定与水体叶绿素a浓度具有最大相关系数的波段组合,然后通过回归方法建立该波段组合与水体叶绿素a浓度的关系模型.在逻辑上,这个流程首先假定了线性关系的存在,然而,实际上测量数据里的波段组合与水体叶绿素a浓度之间的关系经常是非线性关系.针对这个逻辑不一致的问题,提出了一个新的数据处理流程,即
Background Although three-dimensional MRI (3D-MRI) of short T1 inversion recovery turbo spin-echo (STIR TSE) has showed superior to two-dimensional MRI (2D-MRI)
Background The columella,nasal tip,lip relationship in the bilateral cleft lip nasal deformity remains a great challenge for plastic surgeon.An esthetically sat
Background Shoulder immobilization can induce adhesion of the joint,capsular contracture or lead to the condition of frozen shoulder.However,little is known abo
Background The surgical outcomes of decompression for thoracic spinal stenosis (TSS) are unfavorable.The purpose of this study was to determine the efficacy of
Background Most of the basic and clinical studies of osteonecrosis of the femoral head (ONFH) are restricted to bone tissues only,whereas various systems are in