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目的:探讨抗SSA抗体、抗SSB抗体、抗α-fodrin蛋白抗体联合检测在原发性干燥综合征诊断中的应用价值。方法:原发性干燥综合征患者42例(观察组),健康体检者77例(对照组),应用斑点法检测抗SSA抗体、抗SSB抗体,ELISA法检测抗α-fodrin蛋白抗体。结果:抗SSA抗体、抗SSB抗体、抗α-fodrin蛋白抗体在观察组中的阳性率分别为71.4%、40.5%、33.3%,对照组的阳性率分别为15.6%、3.9%、7.8%。抗SSA抗体、抗SSB抗体、抗α-fodrin蛋白抗体在观察组中的特异性分别为84.4%、96.1%、92.2%。观察组抗SSA抗体和抗SSB抗体均阴性者,抗α-fodrin蛋白抗体的阳性率达41.7%。抗SSA抗体、SSB抗体联合抗α-fodrin蛋白抗体检测,可使原发性干燥综合征诊断的阳性率提高至83.3%。结论:抗SSA抗体、抗SSB抗体联合抗α-fodrin蛋白抗体检测,可提高原发性干燥综合征诊断的敏感性。抗SSB抗体及抗α-fodrin蛋白抗体在原发性干燥综合征诊断中均有较高特异性。
Objective: To investigate the value of anti-SSA antibody, anti-SSB antibody and anti-α-fodrin antibody in the diagnosis of Sjogren’s syndrome. Methods: 42 patients with primary Sjogren’s syndrome (observation group) and 77 healthy controls (control group), anti-SSA antibody and anti-SSB antibody were detected by dot blot assay and anti-α-fodrin antibody by ELISA. Results: The positive rates of anti-SSA antibody, anti-SSB antibody and anti-α-fodrin antibody in the observation group were 71.4%, 40.5% and 33.3% respectively. The positive rates in the control group were 15.6%, 3.9% and 7.8% respectively. The specificities of anti-SSA, anti-SSB and anti-α-fodrin antibodies in the observation group were 84.4%, 96.1% and 92.2% respectively. In the observation group, the anti-SSA antibody and anti-SSB antibody were negative, the positive rate of anti-α-fodrin antibody was 41.7%. Anti-SSA antibodies, SSB antibodies combined with anti-α-fodrin antibody detection can make the diagnosis of primary Sjogren’s positive rate increased to 83.3%. Conclusion: Anti-SSA antibody and anti-SSB antibody combined with anti-α-fodrin antibody can improve the diagnostic sensitivity of Sjogren’s syndrome. Anti-SSB antibodies and anti-α-fodrin protein antibodies in the diagnosis of Sjogren’s syndrome have higher specificity.