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目的调查我国广西壮族自治区某食蟹猴养殖场血液寄生原虫感染状况,为人体血液寄生原虫的防控提供科学依据。方法采集广西壮族自治区南宁市某食蟹猴养殖场猴血液样本993份,全部制作FTA卡样本,涂制薄血膜550份。将样本混合,以巢式PCR及普通PCR方法分别检测FTA卡保存的猴血样本中巴贝虫以及疟原虫。检测阳性组再分别进行单个样本检测,阳性样本对应的薄血膜进行姬姆萨染色后再镜检。结果经巢式PCR检测,田鼠巴贝虫检出阳性率为6.95%(69/993);仅1例经PCR检出猪尾猴疟原虫阳性。22份PCR检测巴贝虫阳性的血液样本经染色镜检,共16份检出巴贝虫,其显微镜下观察到红细胞内有环状体,无疟色素。结论我国广西地区食蟹猴的田鼠巴贝虫感染率较高,其可能在传播中起保虫宿主的作用;在筛查田鼠巴贝虫低密度感染时,巢式PCR方法敏感性较高。
Objective To investigate the status of blood parasitic protozoan infection in a cynomolgus monkeys farm in Guangxi Zhuang Autonomous Region of China to provide a scientific basis for the prevention and control of parasitic protozoan blood. Methods 993 monkey blood samples from a cynomolgus monkeys farm in Nanning City of Guangxi Zhuang Autonomous Region were collected, all samples of FTA cards were made and 550 thin films were painted. The samples were mixed to detect Babesia and Plasmodium in monkey blood samples preserved by FTA card by nested PCR and ordinary PCR methods respectively. In the positive group, a single sample was tested separately. The corresponding thin blood film of the positive sample was examined by Giemsa staining. Results The positive rate of Babesia verrucosa detected by nested PCR was 6.95% (69/993). Only one case of Plasmodium vivax was detected by PCR. Twenty-two samples of Pap test were used to detect the positive blood samples of Babesia by Pap test. A total of 16 Babesia were detected, and there were rings in the erythrocytes under the microscope. Conclusion The infective rate of Papilio voles in voles in cynomolgus macaques of Guangxi in China is high, which may play a host role in the transmission. In nested PCR, the nested PCR method is more sensitive in screening low density infection of Babesia microphylla.