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目的:探讨甘草甜素(GL)对HepG2.2.15细胞CCL20表达及细胞存活率的影响。方法:定量RT-PCR检测CCL20的表达,MTT检测细胞的增殖活性,计算细胞存活率,并与空白对照组比较。结果:给药后各组CCL20水平随着剂量的增加而表现为逐渐降低的趋势,与空白对照组比较差异均有统计学意义(P<0.01);MTT实验显示200 mg·L-1以下3个剂量组均可促进细胞增殖(P<0.01);400,800 mg·L-12组均显著抑制细胞增殖(P<0.01)。结论:GL呈剂量依赖抑制CCL20的表达,从而可能抑制HBV引起的免疫炎症反应,但800 mg·L-1组的低表达也可能与细胞的死亡有关。
Objective: To investigate the effect of glycyrrhizin (GL) on the expression of CCL20 and cell viability in HepG2.2.15 cells. Methods: The expression of CCL20 was detected by quantitative RT-PCR. The proliferation activity of cells was assayed by MTT assay. The cell viability was calculated and compared with the blank control group. Results: The levels of CCL20 in each group showed a trend of decreasing gradually with the increase of dose, and the difference was statistically significant compared with the blank control group (P <0.01). The MTT test showed that the level of CCL20 was below 200 mg · L-1 All dose groups could promote cell proliferation (P <0.01), and 400 and 800 mg · L-12 groups significantly inhibited cell proliferation (P <0.01). CONCLUSION: GL inhibits the expression of CCL20 in a dose-dependent manner, which may inhibit the immune-inflammatory reaction induced by HBV. However, the low expression of 800 mg · L-1 may be related to cell death.