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目的分析e抗原定量与HBV-DNA及ALT的相关性,探讨三者之间的关系。方法应用时间分辨荧光免疫分析法和荧光定量PCR法及丙氨酸氨基转移酶法分别对226例乙肝患者血清e抗原和HBVDNA及ALT进行测定,并按e抗原测定结果分成5组。结果①乙肝患者血清e抗原定量与HBV-DNA含量呈等级相关,(相关系数0.514,P<0.001),并且e抗原在0.03～3.75 NCU/ml之间HBV-DNA含量随e抗原定量的升高而升高,而e抗原定量在3.75 NCU/ml以上时与HBV-DNA含量关系不大(Ⅳ组与V组比较P>0.05);②e抗原定量与ALT水平之间不存在相关关系。结论e抗原定量与HBV-DNA含量有很好的一致性,可反映体内HBV的复制情况,但e抗原定量不能代替肝功能的检测,只有同时检测e抗原定量和ALT水平才能为判断HBV感染、复制及肝功能损伤程度提供有价值的诊断依据。
Objective To analyze the relationship between quantitative e antigen and HBV-DNA and ALT, and to explore the relationship between them. Methods The serum e antigen, HBVDNA and ALT were determined in 226 patients with hepatitis B by time-resolved fluorescence immunoassay, fluorescence quantitative PCR and alanine aminotransferase. The e antigen was divided into 5 groups according to the results of e antigen test. Results ① The level of serum e antigen in patients with hepatitis B was correlated with the level of HBV-DNA (correlation coefficient 0.514, P <0.001), and the level of HBV DNA increased with the increase of e-antigen between 0.03 and 3.75 NCU / ml While the level of e antigen was not significantly correlated with the level of HBV-DNA when it was above 3.75 NCU / ml (group Ⅳ vs group V, P> 0.05). ② There was no correlation between e antigen quantitation and ALT level. Conclusion Quantitative e antigen and HBV-DNA content have a good consistency, reflecting the replication of HBV in vivo, but quantitative e antigen can not replace the detection of liver function, only the simultaneous detection of e antigen and ALT levels in order to determine the HBV infection, Replication and liver damage provide a valuable basis for the diagnosis.