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目的 :建立肝得宁 Ⅱ号丸中五味子甲素和五味子乙素的含量测定方法。方法 :高效液相色谱法 ,Kro masilC18分析柱 ,甲醇 四氢呋喃 水 (76∶4∶2 0 )为流动相 ,检测波长 2 5 4nm。结果 :五味子甲素回归方程为Y =2 470 .730X - 1.2 93,r =0 .9999(n =5 ) ,线性范围 0 .1477～ 1.1816 μg ,平均回收率为 97.6 4% ,RSD 1.41% ;五味子乙素回归方程Y =176 2 .32 7X +0 .34 8,r =0 .9997(n =5 ) ,线性范围 0 .0 811～ 0 .6 44 8μg ,平均回收率 96 .14% ,RSD 0 .90 %。结论 :方法简便、快速、准确 ,可作为该制剂的定量方法。
Objective : To establish a method for the determination of schisandrin and schisandrin B in Gandening II pills. Methods: High performance liquid chromatography, Kro masil C18 column, methanol tetrahydrofuran water (76:4:20) as mobile phase, detection wavelength 254nm. Results: The regression equation of schisandrin A was Y = 2 470 .730X - 1.2 93, r = 0.999 (n = 5), the linear range was 0.1477 ~ 1.1816 μg, the average recovery was 97.64%, and RSD was 1.41%. Schizandrin B regression equation Y =176 2 .32 7X +0 .34 8,r =0.9997 (n =5), the linear range was 0 .0 811～ 0 .6 44 8μg, and the average recovery rate was 96.14%. RSD 0 .90 %. Conclusion: The method is simple, rapid, accurate and can be used as a quantitative method for the preparation.