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目的探讨人胎儿胸腺内CD80和癌胚抗原(CEA)的表达水平及定位与胎儿发育的关系。方法自附属医院妇产科收集流产死亡的胎儿12例,其中,小于4个月的4例列为早期,5个月的8例列为晚期。早晚期标本各分为两等份,一份制备冷冻切片进行HE染色、α-醋酸萘酯酶(ANAE)组织化学染色、CD80及CEA免疫组织化学染色,另一份以Trizol提取蛋白后进行CD80的免疫印迹和CEA的酶联免疫吸附测定(ELISA)检测。结果早期胎儿胸腺髓质发育差,晚期胎儿胸腺CD80阳性网状上皮细胞可分为6型:Ⅰ型扁平细胞分布于被膜内面和小梁表面;Ⅱ型呈小星形分布于皮质内;Ⅲ型及Ⅳ型呈扁平状,位于皮髓质交界处;Ⅴ型呈大星形状,突起多而细长相连成网状;Ⅵ型位于哈氏小体。在胎儿胸腺髓质网孔处可见ANAE点型(CD4+CD8-)和散粒型(CD4-CD8+)细胞各呈丛状分布。早期组CEA阳性分布于髓质上皮细胞和哈氏小体,晚期组CEA阳性更集中于哈氏小体。CD80的免疫印迹和CEA的ELISA检测皆显示晚期组表达高于早期组。结论研究结果提示,人胎儿胸腺内CD80和CEA的表达及定位与胎儿胸腺细胞的发育及功能密切相关。
Objective To investigate the relationship between fetal expression of CD80 and carcinoembryonic antigen (CEA) in fetal thymus and fetal development. Methods Twelve fetuses died of miscarriage were collected from obstetrics and gynecology department of the affiliated hospital. Among them, 4 cases less than 4 months were classified as early stage and 8 cases 5 months as late stage. The early and late specimens were divided into two equal parts, one prepared frozen sections for HE staining, α-naphthyl acetate esterase (ANAE) histochemical staining, CD80 and CEA immunohistochemical staining, the other with Trizol protein extraction for CD80 Western blot and CEA enzyme-linked immunosorbent assay (ELISA). Results Early fetal thymus medulla developed poorly. The late fetal thymus CD80 positive reticular epithelial cells could be divided into 6 types: type Ⅰ flat cells distributed on the inner surface of the capsule and the surface of the trabeculae; type Ⅱ showed a small star-shaped distribution in the cortex; type Ⅲ And type Ⅳ was flat, located at the junction of the corticomedullary; Ⅴ type was a large star shape, the protuberances were more and slender connected into a network; Ⅵ type located in the body of Hastelloy. ANAE dot-shaped (CD4 + CD8-) and granular (CD4-CD8 +) cells showed plexiform distribution in fetal thymic medulla mesh. In the early stage, CEA was positive in medullary epithelial cells and Hastellosome, while CEA in late stage was more concentrated in Hastellosome. Immunoblotting of CD80 and CEA ELISA showed that expression of late stage group was higher than that of early stage group. Conclusions The results suggest that the expression and localization of CD80 and CEA in human thymus are closely related to the development and function of fetal thymocytes.