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目的 探讨杭州地区儿童病毒性肠炎的病原和轮状病毒 (RV)分子流行病学特点。方法 用酶联免疫吸附测定 (ELISA)、透射电子显微镜 (TEM)、逆转录聚合酶链反应 (RT PCR)及聚丙烯酰胺凝胶电泳 (PAGE)方法 ,对 1999年 1月 ,在本院就诊的 83例拟诊为急性病毒性肠炎患儿的粪便标本进行了病毒检测和RV组、亚组及血清、基因型测定。结果 RV检出率为 60 2 %( 5 0 / 83 ) ,均为A组、P1A(P[8])基因型 ,主要为Ⅱ亚组 ,G1血清型 ,基因群均为G1P[8]。同时还检测到萼状病毒 (CV)、诺沃克病毒 (NLV)各 3例 ,星状病毒 2例 (此 2例同时检测到RV)。RV检出率以 3～ 2 4个月年龄组最高为 63 2 %( 4 8/ 76) ,RV阳性组的临床表现较阴性组重。结论 1999年 1月杭州地区儿童急性肠炎的主要病原是RV ,属A组、P1A(P[8])基因型、G1P[8]基因群 ,其主要亚组和血清型为Ⅱ亚组、G1血清型。儿童病毒性肠炎存在着多种病原 (除轮状病毒外还有CV、NLV及星状病毒 )并有双重病毒感染存在。RV肠炎好发于 2岁以内婴幼儿 ,且临床表现较非RV感染组重。
Objective To investigate the molecular epidemiology of viral enteritis and rotavirus (RV) in Hangzhou area. Methods ELISA, TEM, RT-PCR and polyacrylamide gel electrophoresis (PAGE) were performed on January 1999 in our hospital 83 cases of patients diagnosed as acute viral enteritis were stool samples for virus detection and RV group, subgroup and serum, genotype determination. Results The detection rate of RV was 60 2% (50/83), both of which were group A and P1A (P [8]) genotypes, mainly Ⅱ subgroup and G1 serotype. All the gene groups were G1P [8]. At the same time, 3 caliciviruses (CV), 3 for Norovirus (NLV) and 2 astrovirus (RV were also detected) were also detected. The detection rate of RV was 63 2% (4 8/76) in the age group of 3 to 24 months, and the clinical manifestations in RV positive group were heavier than those in the negative group. Conclusion The main pathogen of acute enteritis in children in Hangzhou in January 1999 was RV, belonging to group A, P1A (P [8]) and G1P [8]. The major subgroups and serotypes were subgroup Ⅱ, G1 Serotype. There are a variety of viral pathogens in children with viral enteritis (in addition to rotavirus CV, NLV and astrovirus) and the presence of dual virus infection. RV enteritis occurred in infants and young children within 2 years of age, and the clinical manifestations than non-RV infection group weight.