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目的观察弓形虫可溶性速殖子抗原(soluble tachyzoite antigen,STAg)滴鼻、灌胃和皮下注射免疫小鼠诱导的抗弓形虫感染作用。方法40只6~7周龄BALB/c小鼠随机分为4组,前3组分别以20μg STAg滴鼻、灌胃或皮下注射免疫小鼠2次,间隔2周,对照组不做处理。末次免疫后14 d,用速殖子2×104个/只灌胃攻击所有小鼠,观察小鼠健康状况,记录体重,计算存活率。攻虫后30 d颈椎脱位处死小鼠,计数脾和脑组织速殖子。结果滴鼻免疫小鼠的健康状况好于其他组,存活率(70%)高于皮下(60%)、灌胃(60%)和对照组(40%)。滴鼻组脾虫荷为369.48,皮下注射组脾虫荷为386.94,显著低于灌胃组(630.40)和对照组(664.93)(F=3.611,P<0.05)。滴鼻组(36.45)、灌胃组(34.85)和皮下注射组(40.22)脑虫荷差异无统计学意义(F=1.999,P>0.05),但均显著低于对照组(57.32)(F=1.999,P<0.05)。结论20μg STAg滴鼻免疫小鼠诱导了较强的抗弓形虫感染作用,优于皮下和灌胃免疫。
Objective To observe the anti-Toxoplasma infection induced by intranasal, intragastric and subcutaneous injection of soluble tachyzoite antigen (STAg) in mice. Methods Forty BALB / c mice aged 6-7 weeks were randomly divided into four groups. The mice in the first three groups were intranasally injected with 20μg STAg intraperitoneally or intragastrically or subcutaneously twice a week for 2 weeks without any treatment in the control group. At 14 days after the last immunization, all the mice were challenged with 2 × 104 tachyzoites / gavage to observe the health status of the mice, record the body weight, and calculate the survival rate. Mice were sacrificed 30 days after the attack of the cervical spine dislocation, counting tachyzoites in the spleen and brain tissue. Results The intranasal immunization mice were better than the other groups. Survival rate (70%) was higher than subcutaneous (60%), gavage (60%) and control group (40%). 369.48 in the nasal drops group, and 386.94 in the subcutaneous injection group were significantly lower than those in the gavage group (630.40) and the control group (664.93) (F = 3.611, P <0.05). There was no significant difference between the intranasal group (36.45), the intragastric group (34.85) and the subcutaneous injection group (40.22) (P <0.05), but both were significantly lower than that of the control group (57.32) = 1.999, P <0.05). Conclusion 20μg STAg intranasally immunized mice induced strong anti-Toxoplasma infection, better than subcutaneous and gavage immunization.