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目的:观察还脑益聪方对老年认知障碍大鼠学习记忆能力、脑组织炎性因子含量和海马组织核因子-κB(NF-κB)表达的影响。方法:采用自然衰老大鼠模型,应用Morris水迷宫检测,以青年组大鼠平均逃避潜伏期95%和99%正常值范围上限值为界,筛选认知障碍老年大鼠40只。随机分为还脑益聪方大剂量(13.68g生药/kg)组、小剂量(6.84g生药/kg)组、阳性药物对照(盐酸多奈哌齐,0.45mg/kg)组和老年认知障碍大鼠(模型)组,并设立学习记忆正常的青年大鼠10只、正常老年大鼠10只为空白对照组。灌胃给药8周后,观察大鼠学习记忆能力及脑组织白介素-6(IL-6)和肿瘤坏死因子-α(TNF-α)含量,免疫组织化学方法检测海马组织NF-κB的表达。结果:与老年认知障碍大鼠比较,盐酸多奈哌齐组、还脑益聪方组大鼠首次穿越中心的时间显著缩短(P<0.05、P<0.01),在中心区域的寻找平台时间显著加长(P<0.05)。同时,还脑益聪方可以显著降低认知障碍大鼠的IL-6、TNF-α水平(P<0.05,P<0.01),抑制脑组织NF-κB的表达(P<0.05,P<0.01)。结论:还脑益聪方可通过抑制老年认知障碍大鼠的海马组织NF-κB表达,降低脑组织中的炎症因子水平,减轻海马区神经元损伤,达到改善认知障碍大鼠的学习记忆能力。
Objective: To observe the effect of Huannao Yicong Recipe on the learning and memory ability, the content of brain inflammatory factors and the expression of nuclear factor-κB (NF-κB) in the hippocampus of senile rats with cognitive impairment. Methods: A natural aged rat model was used to detect the aged rats with cognitive impairment by using Morris water maze test. The average escape latency of 95% in the young group and the upper limit of 99% normal range were used as the boundary. The rats were randomly divided into two groups: high dose of Huannao YC (13.68g crude drug / kg), low dose (6.84g crude drug / kg), positive drug control (donepezil hydrochloride, 0.45mg / kg) and aged cognitive impairment rats (Model) group, and established a normal learning and memory of 10 young rats, 10 normal aged rats as a blank control group. After 8 weeks of intragastric administration, the learning and memory abilities of rats and the content of IL-6 and TNF-α in brain tissue were observed. The expression of NF-κB in hippocampus was detected by immunohistochemical method . Results: Compared with senile cochlear impaired rats, the time of first pass through center of donepezil hydrochloride and Huannao Yuncong decoction group was significantly shorter (P <0.05, P <0.01), and the time to find platform in central area was significantly longer P <0.05). At the same time, Huannao Yicong Fang can significantly reduce the levels of IL-6 and TNF-α in cognitive impairment rats (P <0.05, P <0.01) and inhibit the expression of NF-κB in brain tissue (P <0.05, P <0.01 ). Conclusion: Huannao Yicong can inhibit the expression of NF-κB in hippocampus of senile cognitive impairment rats, reduce the levels of inflammatory cytokines in the brain tissue, reduce the damage of neurons in the hippocampus and improve the learning and memory of the rats with cognitive impairment ability.