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目的观察亚慢性钐暴露对小鼠肝脏组织结构的影响。方法 80只小鼠随机分成5组,对照组饮用纯净水;实验组分别饮用5、50、500和2 000 mg/L的硝酸钐水溶液,染毒90 d后处死小鼠,迅速取肝脏,准确称重后进行光镜、电镜切片观察肝组织形态学变化。结果与对照组比较,各染毒组小鼠体重无明显差异,2 000 mg/L硝酸钐组肝指数明显低于对照组;光镜检查500 mg/L硝酸钐组肝细胞肿大,轻度变形,胞质疏松肝索肿胀明显,肝窦、中央静脉狭窄,2 000 mg/L硝酸钐染毒细胞排列紊乱,肝细胞边缘模糊,部分肝细胞有破裂现象;电镜检查显示50 mg/L硝酸钐染毒组细胞核核周隙不均匀,核膜曲折不平。线粒体数量减少,粗面内质网增生,有脱颗粒现象,偶见高电子密度致密体,500 mg/L硝酸钐染毒组肝细胞细胞器数量减少,结构模糊,核周粗面内质网消失,糖原颗粒减少,胞质见有多量电子密度高,圆形或椭圆形,大小不等的致密体,2 000 mg/L硝酸钐染毒组细胞核电子密度降低,胞质中有较多密度较高,体积较小致密体,核旁可见脂滴,线粒体电子密度较低,结构不清晰,内质网扩张。结论高剂量硝酸钐对肝结构具有损害作用,提示肝脏是稀土元素钐作用的靶器官。
Objective To observe the effects of subchronic samarium exposure on the liver tissue of mice. Methods 80 mice were randomly divided into 5 groups, the control group drinking pure water; experimental group were drinking 5,50,500 and 2 000 mg / L of samarium nitrate solution, 90 days after the mice were sacrificed, quickly take the liver, accurate After weighing, light microscopy and electron microscopy were performed to observe the morphological changes of liver tissue. Results Compared with the control group, there was no significant difference in the body weight of mice exposed to 2 000 mg / L samarium nitrate. The liver index of 500 mg / L samarium nitrate group was significantly higher than that of the control group Deformation, loose cytoplasm obvious hepatic cord swelling, sinusoidal, central venous stenosis, 2 000 mg / L samarium nitrate poisoned cells arranged disorders, fuzzy edge of liver cells, some hepatocytes rupture; electron microscopy showed 50 mg / L nitric acid Samarium exposure group nuclear peritumoral uneven, nuclear membrane tortuous. Mitochondria decreased, rough endoplasmic reticulum hyperplasia, degranulation phenomenon, occasionally high electron density dense body, 500 mg / L samarium nitrate decreased the number of hepatocytes organelles, fuzzy structure, perinuclear rough endoplasmic reticulum disappeared , Glycogen particles decreased, the cytoplasm to see a large number of high electron density, round or oval, the size of the dense body, 2 000 mg / L samarium nitrate cell nucleus electron density decreased, the cytoplasm more density Higher, smaller volume dense body, visible lipid droplets near the nucleus, mitochondrial electron density is low, the structure is not clear, the endoplasmic reticulum dilatation. Conclusion High doses of samarium nitrate have a damaging effect on the liver structure, suggesting that the liver is the target organ of samarium.