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目的:建立快速、准确、灵敏测定海产品中甲醛的新方法。方法:在沸水浴中,痕量甲醛与乙酰丙酮能发生微弱的显色反应。实验发现,非离子表面活性剂OP对上述反应有较强的增敏作用,由此建立了一种测定痕量甲醛的新方法。结果:在测定条件下,溶液的最大吸收波长在412 nm,方法的检出限为0.11μg/ml,相关系数为0.9993,九次空白实验的标准偏差为S=0.00365,相对标准偏差为RSD为0.376%,回收率在100%～105%。结论:方法简单快速、选择性好,用于测定海产品中的甲醛时,测得结果与非增敏乙酰丙酮光度法及HPLC法的结果一致。
Objective: To establish a new method for the rapid, accurate and sensitive determination of formaldehyde in seafood. Methods: In a boiling water bath, traces of formaldehyde and acetylacetone weak color reaction occurs. The experiment found that non-ionic surfactant OP has a strong sensitizing effect on the above reaction, thus establishing a new method for the determination of trace formaldehyde. Results: Under the conditions of the determination, the maximum absorption wavelength of the solution was at 412 nm. The detection limit was 0.11μg / ml. The correlation coefficient was 0.9993. The standard deviation of the blank test was S = 0.00365. The relative standard deviation was RSD 0.376%, recovery rate of 100% ~ 105%. Conclusion: The method is simple, rapid and selective. When used for the determination of formaldehyde in seafood, the results are consistent with the results of non-sensitized acetylacetone spectrophotometry and HPLC.