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目的:观察米诺环素(minocycline,MC)对癫痫海马小胶质细胞P2X7表达的作用。方法:原代培养大鼠海马小胶质细胞并建立海人酸(kainicacid,KA)损伤模型,并分组:空白对照组、KA对照组、米诺环素处理组,观察各组细胞形态学的变化,检测各组P2X7受体mRNA的表达变化。结果:经KA处理后,海马小胶质细胞从静息状态变为激活状态,而米诺环素处理可以抑制小胶质细胞形态学的改变;经KA处理后,P2X7受体mRNA表达增多,与空白对照组比,升高值为1.825±0.24倍,有统计学意义(P<0.05);而米诺环素处理组,P2X7受体mRNA表达量为1.114±0.09倍,与KA组比较,有明显的统计学意义,P<0.01。结论:米诺环素能有效抑制癫痫海马小胶质细胞的活化,减少P2X7受体的表达。
Objective: To observe the effect of minocycline (MC) on the expression of P2X7 in epileptic hippocampal microglia. Methods: Primary cultured rat hippocampal microglia cells were established and the kainic acid (KA) injury model was established. The rats were divided into blank control group, KA control group and minocycline treatment group. Morphological Changes, detection of P2X7 receptor mRNA expression changes in each group. Results: After KA treatment, the microglia of hippocampus changed from rest state to activation state, while minocycline treatment could inhibit the morphological changes of microglia cells. After KA treatment, the expression of P2X7 receptor mRNA increased, Compared with the blank control group, the increase value was 1.825 ± 0.24 times, which was statistically significant (P <0.05). Compared with the KA group, the mRNA expression of P2X7 receptor in the minocycline group was 1.114 ± 0.09 times, Significant statistical significance, P <0.01. Conclusion: Minocycline can effectively inhibit the activation of microglia in the hippocampus and decrease the expression of P2X7 receptor in epileptic rats.