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以成熟种子和胚轴为外植体诱导草地早熟禾愈伤组织,比较草地早熟禾四个品种的愈伤诱导情况和不同6-BA浓度、愈伤年龄等因素对愈伤组织分化能力的影响。结果表明:成熟种子的出愈率与胚性愈伤诱导率均高于胚轴;MS+2,4—D(2mg/L)+6-BA(0.1mg/L)培养基为草地早熟禾Mardona品种较为合适的愈伤培养基,其诱导率为58.3%;MS+BA(3mg/L)+KT(0.2mg/L)为较为适合的分化培养基,再生率高达70%;随着继代次数的增加,草地早熟禾分化能力能够继续保持。选择致密、易碎、生长迅速的愈伤继代能够保持草地早熟禾的胚性,可以为遗传转化提供长期良好的植物材料。
The effects of callus induction and different concentrations of 6-BA and callus age on the callus differentiation of four cultivars were studied by using mature seeds and hypocotyls as explants. . The results showed that the callus induction rate and embryogenic callus induction rate of mature seeds were higher than that of hypocotyls. The medium of MS + 2,4-D (2mg / L) + 6-BA (0.1mg / L) Mardona is the most suitable callus culture medium with the induction rate of 58.3%. MS + BA (3mg / L) + KT (0.2mg / L) is the most suitable culture medium with the regeneration rate as high as 70% With the increase of generation times, the ability of P. olaevocum to differentiate can continue. The selection of dense, fragile and rapidly growing callus, which maintains the embryogenic nature of Kentucky Bluegrass, provides long-term good plant material for genetic transformation.