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目的：探讨心理应激源对脑组织Ｎａ＋ ，Ｋ＋ － ＡＴＰ酶活性的影响及发生机制，以及自由基清除剂对该酶活性的保护作用。方法：将大鼠分为对照组、束缚组和治疗组。用酶组化法制片，用图像分析仪测脑组织Ｎａ＋ ，Ｋ＋ － ＡＴＰ酶的活性；用ＴＡＢ法测ＭＤＡ的含量。结果：束缚组和治疗组的Ｎａ＋ ，Ｋ＋ － ＡＴＰ酶活性明显低于对照组，ＭＤＡ含量明显高于对照组；治疗组Ｎａ＋ ，Ｋ＋ － ＡＴＰ酶活性明显高于束缚组，ＭＤＡ含量明显低于束缚组；束缚组、治疗组的ＭＤＡ含量与Ｎａ＋ ，Ｋ＋ － ＡＴＰ酶活性呈直线负相关。结论：较长时间的束缚应激可致大鼠脑组织Ｎａ＋ ，Ｋ＋ － ＡＴＰ酶活性降低；该酶活性降低与氧自由基损伤有关；给予ＳＯＤ＋ ＣＡＴ治疗，对该酶活性具有一定的保护作用
Objective: To investigate the effect of psychological stressors on the activity of Na +, K + - ATPase in brain tissue and its mechanism, and the protective effect of free radical scavenger on the activity of Na +, K + - ATPase. Methods: The rats were divided into control group, restraint group and treatment group. The activity of Na +, K + - ATPase in the brain tissue was determined by image analysis and the content of MDA was measured by TAB method. Results: The activities of Na +, K + - ATPase in shackles group and treatment group were significantly lower than those in control group, while the content of MDA in shackle group and treatment group was significantly higher than those in control group. The activities of Na +, K + - ATPase in treatment group were significantly higher than those in shackles group Group. The contents of MDA and the activities of Na +, K + - ATPase in the binding group and the treatment group showed a linear negative correlation. CONCLUSION: The prolonged restraint stress can decrease the activity of Na +, K + - ATPase in rat brain. The decrease of the activity is related to the damage of oxygen free radicals. The treatment with SOD + CAT has certain protective effect on the activity