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利用电化学氧化的方法制备了水溶性好、粒径为7～12nm的碳纳米粒子,该碳纳米粒子通过π-π相互作用吸附荧光标记的单链DNA探针,并能有效地猝灭其荧光.当单链DNA探针与匹配的DNA目标分子杂交形成双链DNA时,猝灭的荧光被恢复,由此可以检测1～200nmol/L的DNA目标分子.此外,在碳纳米粒子存在时,由荧光标记的DNA探针和DNA目标分子形成的双链DNA的熔解温度可以简便地被测定,当双链DNA有错配碱基时,其熔解温度降低,由此可方便、快速地分析单核苷酸多态性.
Electrochemical oxidation method was used to prepare water-soluble, particle size of 7 ~ 12nm carbon nanoparticles, the carbon nanoparticles by π-π interaction fluorescence-labeled single-stranded DNA probe, and can effectively quench Fluorescence When the single-stranded DNA probe hybridizes to a matched DNA target to form double-stranded DNA, the quenched fluorescence is restored, whereby a DNA target molecule of 1 to 200 nmol / L can be detected In addition, in the presence of carbon nanoparticles , The melting temperature of the double-stranded DNA formed by the fluorescently labeled DNA probe and the DNA target molecule can be easily measured. When the double-stranded DNA has a mismatch base, its melting temperature is lowered, whereby the analysis can be conveniently and quickly performed Single nucleotide polymorphism.