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应用卡尔斯伯格种子固定装置把大麦籽粒嵌入陶瓷板上的籽粒槽中,擦破皮壳以暴露一部分胚乳及其周围的皮壳。然后把籽粒浸入含有4.5当量盐酸的香草醛溶液中,对皮壳中的原花色素进行染色。把没有原花色素的籽粒挑选出来,洗净并将其培育至成熟.利用叠氮化钠诱变,在78,450粒 M_3代籽粒中获得了10株无原花色素的突变体,这些突变体的营养器官含有花色素苷的颜色,其突变频率是0.013%。
Barley kernels were embedded in the grain slots on a ceramic plate using a Karlström seed holder, and the scallops were rubbed to expose a portion of the endosperm and the surrounding scallops. The seeds were then dipped in a vanillin solution containing 4.5 equivalents of hydrochloric acid to stain the proanthocyanidin in the crust. The seeds without proanthocyanidins were selected, washed and cultivated to maturity.Using sodium azide mutagenesis, 10 proanthocyanidin-free mutants were obtained from 78,450 M_3 seeds, Vegetative organs contain the color of anthocyanins with a mutation frequency of 0.013%.