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目的 :探讨磷酸己糖异构酶 (GPI)在肿瘤诊断及病情监测中的临床应用价值。方法 :采用改良的化学比色法测定GPI活性。结果 :对 718例血清标本GPI活性测定发现 ,恶性肿瘤组血清GPI活性( 149.2 6± 75.2 3)IU/L显著高于健康对照组 ( 53.96± 2 3.78)IU/L和良性疾病组 ( 75.93± 35.67)IU/L ,P <0 .0 5。肺癌、肝癌、胰腺癌和胆囊癌的检测阳性率分别为 88 4 6% ,83 87% ,82 61%和 79 31% ,明显高于其他肿瘤组、良性疾病组和正常对照组 (P <0 .0 5)。进展期及出现远处转移的肿瘤患者血清GPI活性 ( 197.2 6± 93.74 )IU/L明显高于缓解期患者 ( 84 .57± 4 3.38)IU/L ,P <0 0 1。经手术、放疗或化疗后患者血清GPI活性明显降低 ,尤以手术后GPI活性降低最为明显。结论 :①GPI活性检测可用于恶性肿瘤尤其是肺癌、肝癌、胰腺癌和胆囊癌的辅助诊断和病情监测 ;②改良的GPI活性测定方法简便、快速、稳定、经济 ,便于在基层单位推广
Objective: To explore the clinical application value of phosphoenolpyruvate isomerase (GPI) in the diagnosis and monitoring of the disease. Methods : GPI activity was measured by modified chemical colorimetry. RESULTS: The GPI activity in 718 serum samples was found to be significantly higher in serum GPI activity (149.2 6±75.2 3) IU/L than in the healthy control group (53.96±2 3.78) IU/L and benign disease group (75.93±). 35.67) IU/L, P <0. The positive rates of lung cancer, liver cancer, pancreatic cancer and gallbladder cancer were 88 4 6%, 83 87%, 82 61% and 79 31%, respectively, which were significantly higher than other tumor groups, benign disease groups and normal controls (P < 0 .0 5). Serum GPI activity (197.2 6± 93.74) IU/L was significantly higher in patients with advanced metastases and distant metastases than those in remission (84.57±4 3.38) IU/L, P <0 01. After surgery, radiotherapy or chemotherapy, the serum GPI activity was significantly reduced, and the GPI activity decreased most significantly after surgery. Conclusion : 1GPI activity test can be used for the diagnosis and monitoring of malignant tumors, especially lung cancer, liver cancer, pancreatic cancer and gallbladder cancer. 2The improved GPI activity measurement method is simple, rapid, stable and economical, and it is convenient for promotion in grass-roots units.