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6、血清溶菌酶活性的测定以溶壁微球菌(M icrococcuslysoleikticus)冻干粉为底物,将底物用0.1mol/L的pH为6.4的磷酸钾盐缓冲液配成一定浓度的悬液(O.D.570≈0.35￣0.5)。取3.0mL该悬液与0.05mL待测血清于试管中混匀,测其在570nm处的光密度值(A0),然后置于37℃水浴保温30min,取
6, the determination of serum lysozyme activity Micrococcus lysolyticus (M icrococcus lysoleikticus) lyophilized powder as a substrate, the substrate with 0.1mol / L pH 6.4 of potassium phosphate salt buffer dubbed a certain concentration of the suspension ( OD570≈0.35 ~ 0.5). Take 3.0mL of the suspension and 0.05mL serum to be tested in the tube mix, measured at 570nm optical density value (A0), and then placed in 37 ℃ water bath incubated 30min, take