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目的:建立同时测定地奥心血康胶囊中原薯蓣皂苷、甲基原薯蓣皂苷和薯蓣皂苷3种成分含量的HPLC-ELSD分析方法。方法:采用Shim-pack VP-ODS色谱柱(250mm×4.6mm,5μm),以乙腈(A)-水(B)(0～15min,B:75%→71%;15～25min,B:71%;25～45min,B:71%→65%;45～65min,B:65%→40%;66min,B:30%;66～75min,B:30%→0%)为流动相进行梯度洗脱,流速为0.8mL·min-1,柱温为35℃。采用蒸发光散射检测器,漂移管的温度为105℃,气体(空气)流速为2.7L·min-1。结果:原薯蓣皂苷、甲基原薯蓣皂苷和薯蓣皂苷的线性范围分别为1.021～10.21μg(r=0.9999),1.029～10.29μg(r=0.9995)和1.074～10.74μg(r=0.9998),平均回收率(n=9)依次为99.2%(RSD=2.0%),101.9%(RSD=1.5%)和100.7%(RSD=2.0%)。结论:本方法简便、准确、重现性好,可用于地奥心血康胶囊中原薯蓣皂苷、甲基原薯蓣皂苷和薯蓣皂苷的含量测定。
OBJECTIVE: To establish a HPLC-ELSD method for the simultaneous determination of three components, Diosgenin, Methyl diosgenin and Diosgenin in Dioxin Xingshikang Capsules. Methods: The chromatographic separation was performed on a Shim-pack VP-ODS column (250 mm × 4.6 mm, 5 μm) using acetonitrile (A) -water (B) %; 25-45 min, B: 71% → 65%; 45-65 min, B: 65% → 40%; 66 min, B: 30%; 66-75 min, B: 30% → 0%) as the mobile phase Eluted at a flow rate of 0.8mL · min-1, the column temperature was 35 ℃. Using an evaporative light scattering detector, the drift tube temperature was 105 ° C and the gas (air) flow rate was 2.7 L · min-1. Results: The linear range of the original diosgenin, methyl original diosgenin and diosgenin were 1.021-10.21μg (r = 0.9999), 1.029-10.29μg (r = 0.9995) and 1.074-10.74μg (r = 0.9998) The recoveries (n = 9) were 99.2% (RSD = 2.0%), 101.9% (RSD = 1.5%) and 100.7% (RSD = 2.0%) in turn. Conclusion: The method is simple, accurate and reproducible. It can be used for the determination of original diosgenin, methyl protodioscin and diosgenin in Dioxin Xueshikang Capsules.