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作者为研究日本血吸虫感染鼠发病过程中体液(血清)和细胞免疫调节机制的作用,分别用感染了血吸虫10、20、30周的鼠血清、脾细胞和脾中T细胞转入感染了血吸虫4～5周的鼠体内,观察在这些因素影响下,炎性肉芽肿和门脉压的动态变化。取C57BL/6雌性鼠,重18～22g,腹腔注射日本血吸虫菲律宾株尾蚴25条,与未感染鼠分别作为实验血清和细胞供体。有些实验血清经A蛋白-琼脂糖凝胶柱层析,分离出,IgM、IgA、IgG_1、IgG_(2a)、IgG_(2b)/IgG_3,经微量琼脂扩散鉴定纯度后备用,摘取脾脏,用PBS制成纯脾细胞悬液,将脾细胞于尼龙柱上培养(37℃、30分钟),分离出T细胞。每只试验观察鼠也感染尾蚴25条,4周后分组,
In order to study the role of humoral (serum) and cellular immune regulatory mechanisms in the pathogenesis of Schistosoma japonicum infection in mice, the T cells infected with Schistosoma japonicum 4 ~ 5 weeks in vivo, the dynamic changes of inflammatory granuloma and portal pressure were observed under the influence of these factors. C57BL / 6 female mice weighing 18-22g were injected intraperitoneally with 25 strains of S. japonicum cercariae, which were used as experimental serum and cell donor, respectively. Some of the experimental sera were purified by Protein A-Sepharose column chromatography, and IgM, IgA, IgG1, IgG2 (2a) and IgG2 (2b) / IgG3 were separated and identified by micro agar diffusion. Pure spleen cell suspension was prepared by PBS. Splenocytes were cultured on nylon column (37 ° C, 30 minutes), and T cells were isolated. Each experiment also observed mice infected cercariae 25, 4 weeks after grouping,