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目的探讨白藜芦醇(Res)对培养的糖尿病大鼠内皮祖细胞(EPCs)生物学功能的影响。方法采用链脲佐菌素(STZ)腹腔注射联合高糖高脂饮食方法,建立2型糖尿病(T2DM)大鼠模型。造模成功后,Histopaque-1083分离大鼠骨髓单核细胞,种植于EGM-2MV条件培养基中。不同浓度的Res(0,10,25,50μmol/L)孵育第3~5代EPCs 24h,分别采用Edu标记技术、黏附能力测定实验、改良的Boyden小室检测EPCs的增殖、黏附和迁移能力。ELISA法检测EPCs上清液中单核细胞趋化蛋白-1(MCP-1)含量。结果与正常对照组相比,糖尿病大鼠EPCs的增殖、黏附及迁移能力均明显降低,而炎症因子MCP-1的分泌增加;Res可显著提高EPCs的增殖、黏附和迁移能力,同时下调MCP-1的分泌。结论糖尿病大鼠EPCs的生物学功能受损,Res能明显改善糖尿病大鼠EPCs的功能,为临床防治糖尿病心血管并发症提供了理论及实验依据。
Objective To investigate the effect of resveratrol on the biological function of endothelial progenitor cells (EPCs) in cultured diabetic rats. Methods The model of type 2 diabetes mellitus (T2DM) was established by intraperitoneal injection of streptozotocin (STZ) combined with high glucose and high fat diet. After successful modeling, rat bone marrow mononuclear cells were isolated from Histopaque-1083 and seeded in EGM-2MV conditioned media. EPCs of passage 3-5 were incubated with various concentrations of Res (0, 10, 25 and 50μmol / L) for 24h. The proliferation, adhesion and migration of EPCs were detected by Edu labeling assay, adhesion assay and modified Boyden chamber respectively. The levels of monocyte chemoattractant protein-1 (MCP-1) in EPCs supernatants were detected by ELISA. Results Compared with the normal control group, the proliferation, adhesion and migration of EPCs in diabetic rats were significantly decreased, while the secretion of inflammatory cytokines MCP-1 increased; Res significantly increased the proliferation, adhesion and migration of EPCs, 1 secretion. Conclusion The biological function of EPCs in diabetic rats is impaired. Res can significantly improve the function of EPCs in diabetic rats, which provides a theoretical and experimental basis for clinical prevention and treatment of diabetic cardiovascular complications.