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目的 用电子自旋共振 (electron spin resonance,ESR)技术观察模拟飞行缺氧条件下小鼠不同脏器一氧化氮 (nitric oxide,NO)含量的变化及抗氧化剂茶多酚的防护作用。 方法 昆明种雄性小鼠 2 1只 ,随机分为 3组 (n=7) :地面对照组 ;15 0 0 m轻度低压缺氧组 ;15 0 0 m轻度低压缺氧 +茶多酚防护组。其中茶多酚防护组在低压暴露前以 10 0 mg/ kg剂量灌胃 (其余两组灌蒸馏水 ) ,然后与单纯缺氧组一起置于低压舱中。低压舱条件为 :模拟高度 15 0 0 m ,上升速率 30 m/ s,下降速率15 m/ s,暴露时间为 2 h/ d,每周 3d,共 8周。末次低压舱处理完毕后 ,于次日将小鼠断头处死 ,迅速取脑、心、肺、肝、肾各约 2 0 mg,血 30μl,置于 2 0μl的 (MGD) 2 Fe2 + 生理盐水溶液中 ,室温浸泡 30 m in。检测各组织及血液 ESR强度 ,并计算出相应的 NO含量。 结果 15 0 0 m轻度低压缺氧 8周后 ,与地面对照组相比 ,肺组织 NO含量显著增高 (P<0 .0 1) ,肾组织 NO含量明显降低 (P<0 .0 5 )。用茶多酚防护后肺、肝 NO含量较单纯缺氧组明显降低 (P<0 .0 1、P<0 .0 5 ) ,而肾组织 NO含量有增加趋势 ,但无统计学差异。 结论 15 0 0 m轻度低压缺氧 8周 ,肺组织 NO含量代偿性增高 ,而肾组织NO含量降低 ,茶多酚对这种效应有一定的?
Objective To observe the changes of nitric oxide (NO) in different organs and the protective effect of antioxidant tea polyphenols on simulated flight hypoxia using electron spin resonance (ESR) technique. Methods Twenty-one Kunming male mice were randomly divided into three groups (n = 7): ground control group, mild hypobaric hypoxia group of 150 m, hypobaric hypoxia of 150 m and protective effect of tea polyphenols group. The tea polyphenols protective group was given gavage at the dose of 100 mg / kg before the low pressure exposure (the other two groups were filled with distilled water), and then placed in the low pressure cabin together with the simple hypoxia group. The conditions of the low-pressure cabin are as follows: the simulated altitude is 150 m, the rate of ascent is 30 m / s, the rate of decline is 15 m / s, the exposure time is 2 h / d, and 3d is weekly. After the last low-pressure tank treatment, the mice were decapitated on the next day, and about 20 mg of brain, heart, lung, liver and kidney were rapidly taken and 30 μl of blood were placed in 20 μl of (MGD) 2 Fe2 + In aqueous solution, soak for 30 mins at room temperature. The ESR intensity of each tissue and blood was measured and the corresponding NO content was calculated. Results Compared with the ground control group, the content of NO in the lung tissue was significantly increased (P <0.01) and the NO content in the kidney tissue was significantly decreased (P <0.05) after mild hypobaric hypoxia for 15 weeks. . Compared with simple hypoxia group, the content of NO in lung and liver after protection by tea polyphenols was significantly decreased (P <0.01, P <0.05), while the content of NO in kidney tissue had an increasing tendency, but there was no statistical difference. CONCLUSION: After hypoxia for 15 minutes and mild hypobaric hypoxia for 8 weeks, the content of NO in lung tissue is compensatory, while the content of NO in kidney tissue is decreased. The effect of tea polyphenols on this effect is certain.