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目的:比较正常肝组织与肝癌AH 109A,吉田肉瘤中谷氨酸脱氢酶,胆碱氧化酶和葡萄糖-6-磷酸酶的活力对~(67)Ga摄取与积累的影响;方法:制备~(67)Ga枸橼酸溶液给大鼠静注后处死大鼠,制备亚细胞悬液,液闪计数器测定放射活度.结果:~(67)Ga的放射活性在正常肝组织溶酶体中(55％积聚)显著高于肝癌AH109A(32％积聚)和吉田肉瘤(18％)积聚.谷氨酸脱氢酶的活力在正常肝组织,肝癌和吉田肉瘤分别是1830±s 320 U·L~(-1),23±s 6 U·L~(-1)和7±s 2 U·L~(-1);胆碱氧化酶的活力分别是46±s 10 U·L~(-1),25.0±s 0.4 U·L~(-1),2.0±0.4 U·L~(-1);葡萄糖-6-磷酸酶活力分别是2550±s 180 U·L~(-1),84±s 14 U·L~(-1),78±s13 U·L~(-1).结论:正常肝组织中溶酶体酶活力很强,对~(67)Ga的积累起较大作用.癌变组织酶活力降低而作用减弱.吉田肉瘤细胞无肝细胞特点,其溶酶体对~(67)Ga积累作用不大.
OBJECTIVE: To compare the effects of glutamate dehydrogenase, choline oxidase and glucose-6-phosphatase activity on the uptake and accumulation of ~(67)Ga in normal liver tissue and liver cancer AH 109A, and Yoshida sarcoma; 67) Ga citrate solution was administered to rats after intravenous injection to prepare a subcellular suspension. The radioactivity was measured with a liquid scintillation counter. Results: Radioactivity of ~(67)Ga in normal liver tissue lysosomes ( The accumulation of 55% was significantly higher than that of liver cancer AH109A (32% accumulation) and Yoshida sarcoma (18%). The activity of glutamate dehydrogenase was in normal liver tissue, and the liver cancer and Yoshida sarcoma were 1830±s 320 U·L~, respectively. (-1), 23±s 6 U·L~(-1) and 7±s 2 U·L~(-1); the viability of choline oxidase was 46±s 10 U·L~(-1), respectively. ), 25.0±s 0.4 U·L~(-1), 2.0±0.4 U·L~(-1); glucose-6-phosphatase activity was 2550±s 180 U·L~(-1), 84 ±s 14 U·L~(-1), 78±s13 U·L~(-1). Conclusion: The activity of lysosomal enzymes in normal liver tissue is very strong, which plays a significant role in the accumulation of ~(67)Ga. The enzyme activity of cancer tissue decreased and the effect was weakened. Yoshida sarcoma cells had no characteristics of hepatocytes, and their lysosomes had little effect on ~(67)Ga accumulation.