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目的：研究ＣＤ５＋Ｂ细胞在重症肌无力（ＭＧ）发病中的作用。方法：采用免疫荧光双标记技术和流式细胞仪对３９例ＭＧ患者和１８例健康对照者周围血中ＣＤ５＋Ｂ细胞（ＣＤ５＋ＣＤ１９＋）的百分率进行测定，同时用ＥＬＩＳＡ间接法检测ＭＧ患者血清中ＡＣｈＲａｂ。结果：在ＭＧ患者周围血ＣＤ５＋Ｂ细胞百分率显著高于对照组，而且在血清ＡＣｈＲａｂ阳性和阴性ＭＧ中ＣＤ５＋Ｂ细胞均明显高于对照组；但在血清ＡＣｈＲａｂ阳性和阴性ＭＧ患者之间ＣＤ５＋Ｂ细胞百分率无显著性差异发现。结论：ＣＤ５＋Ｂ细胞与ＭＧ发病有关，但与ＡＣｈＲａｂ的产生之间无显著相关。
Objective: To study the role of CD5 + B cells in the pathogenesis of myasthenia gravis (MG). Methods: The percentage of CD5 + B cells (CD5 + CD19 +) in peripheral blood of 39 patients with MG and 18 healthy controls were measured by immunofluorescence double labeling and flow cytometry. Serum AChRab was detected by ELISA indirect ELISA. Results: The percentage of CD5 + B cells in peripheral blood of patients with MG was significantly higher than that in control group, and CD5 + B cells were significantly higher in AChRab-positive and -negative MG serums than in control group; however, there was no significant difference in the percentage of CD5 + B cells between AChRab positive and negative MG patients Sex differences found. Conclusion: CD5 + B cells are associated with the pathogenesis of MG but not with AChRab.