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采用免培养技术对河西走廊酒泉地区的原生盐碱土、次生盐碱土和农田土中的放线菌群落结构及其多样性进行分析.结果表明:河西走廊酒泉地区原生盐碱土克隆文库分归于19个OTUs,分属于微球菌亚目、丙酸杆菌亚目、棒状杆菌亚目、弗兰克氏菌亚目、假诺卡氏菌亚目和放线菌目未知类群;次生盐碱土克隆文库分归于14个OTUs,分属于微球菌亚目、丙酸杆菌亚目和放线菌目未知类群;农田土克隆文库分归于7个OTUs,分属于微球菌亚目和棒状杆菌亚目;微球菌亚目是3种不同类型土壤中的共有种群,是原生盐碱土和农田土中的优势种群.多样性指数和稀释性曲线分析结果显示,3种不同类型土壤中放线菌种群丰富度为原生盐碱土>次生盐碱土>农田土;原生盐碱土、次生盐碱土的稀释性曲线均未趋于平稳,说明盐碱土中放线菌多样性比实际更加丰富.
Using culture-free culture, the actinomycete community structure and its diversity in primary saline soil, secondary saline soil and agricultural soil in Jiuquan area of Hexi Corridor were analyzed.The results showed that the clonal libraries of native saline-alkali soil in Jiuquan area of Hexi Corridor belonged to 19 OTUs belonging to Micrococcus subfamily, Propionibacterium subtilis, Corynebacterium subfamily, Frankincense subfamily, Pseudonocardia subfamily and Actinomycetes unknown group; secondary salinization soil clone library attribution to 14 OTUs belonged to Micrococcus subgroups, Propionibacteria subgroups and Actinomycetes unknown groups; TOC library was divided into 7 OTUs, belonging to Micrococcus subtilis and Corynebacterium subfamilies; Is the common population of three different types of soils and is the dominant species in native saline soil and farmland soil.The results of diversity index and dilution curve showed that the population richness of actinomycetes in three different types of soils was primary salt Alkaline earth> secondary saline-alkaline soil> farmland soil. The dilution curves of primary saline-alkali soil and secondary saline-alkali soil did not tend to be stable, indicating that the diversity of actinomycetes in saline-alkali soil was more abundant than the actual ones.