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目的观察蜂眠宁胶囊对D-半乳糖致衰老模型小鼠的抗衰老作用,为蜂眠宁胶囊预防和治疗老年痴呆症提供临床依据。方法用D-半乳糖制备小鼠衰老模型,采用Morris水迷宫进行定位航行试验、空间搜索试验,测定各组小鼠的逃避潜伏期及穿越平台次数;并测定各组小鼠组织中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性和丙二醛(MDA)含量。结果与模型组相比,蜂眠宁胶囊低剂量组(0.6g·kg-1·d-1)、高剂量组(0.9g·kg-1·d-1)逃避潜伏期均明显减少(P<0.05),且穿越平台次数均显著增多(P<0.05,P<0.01);蜂眠宁胶囊高剂量组(0.9g·kg-1·d-1)能显著提升D-半乳糖所致衰老小鼠脑组织中SOD、GSH-Px的活性,降低脑组织中MDA的含量。结论蜂眠宁胶囊能改善D-半乳糖致衰老模型小鼠的学习记忆能力,抑制其脑组织脂质过氧化,具有抗衰老作用。
Objective To observe the anti-aging effect of beeoningning capsule on aging mice induced by D-galactose, and to provide a clinical basis for the prevention and treatment of Alzheimer’s disease by beeoningning capsule. Methods The mouse model of aging was established by using D-galactose. Morris water maze was used to conduct navigation and space search test to determine the escape latency and the number of traversing platforms. The levels of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) activity and malondialdehyde (MDA) content. Results Compared with the model group, the escape latency of Beomuoning Capsule low dose group (0.6g · kg -1 · d -1) and high dose group (0.9g · kg -1 · d -1) were significantly decreased (P < (P <0.05, P <0.01). The high dose of Beomuoning Capsule (0.9g · kg-1 · d-1) significantly increased the number of senescence induced by D-galactose The activity of SOD and GSH-Px in brain tissue of rats decreased and the content of MDA in brain tissue decreased. Conclusion Beomianning capsule can improve the learning and memory abilities of aging mice induced by D-galactose and inhibit the lipid peroxidation of brain tissue of mice with anti-aging effect.