本文报道用高效液相色谱法测定血清尿酸,测定是在反相 C_(18)柱上进行,0.01M 醋酸铵∶甲醇=100∶7(v/v)作流动相,紫外检测器检测,波长280nm,分离的尿酸用两点外标法定量,以峰面积对尿酸浓度作标准曲线显示相关系数为0.9987,平均回收率为100.17％,变异系数<0.06％。本文着重讨论了流动相的选择。 This paper reports the determination of serum uric acid by high performance liquid chromatography. The determination was carried out on reversed-phase C_ (18) column with 0.01M ammonium acetate: methanol = 100: 7 (v / v) 280nm, uric acid isolated by two external standard method to quantify the concentration of uric acid peak area for the standard curve showed a correlation coefficient of 0.9987, the average recovery was 100.17%, coefficient of variation <0.06%. This article focuses on the choice of mobile phase.