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在以前研究中作者证明经照射的、分泌IL-2的MBT-2细胞制剂能治愈正位建立的(orthotopically established)小鼠膀胱癌,并引起保护性免疫记忆。本次研究中作者探讨了IL-1α,IL-1β,IFN-γ和GM-CSF修饰的肿瘤细胞的治疗效果,并与IL-2进行了比较;探讨治疗鼠CTL诱导与细胞因子基因修饰的肿瘤细胞制剂疗效的关系。 方法:①MBT-2细胞正位植入C_3H/He、Swiss nu/nu小鼠膀胱:戊巴比妥麻醉,暴露膀胱, MBT-2细胞+50μl PBS注入膀胱壁内。②逆转录病毒载体设计和分泌细胞因子的MBT-2细胞系制备:逆转录病毒载体来自Moloney鼠白血病病毒。细胞因子cDNA融合到载体中的启动子上。GM-CSF,IL-1α,IL-1β,IFN-γcDNA分别克隆到PBC140,DC/
In previous studies, the authors demonstrated that irradiated, IL-2-secreting MBT-2 cell preparations can cure orthotopically established mouse bladder cancer and cause protective immune memory. In this study, the authors investigated the therapeutic effect of IL-1α, IL-1β, IFN-γ and GM-CSF modified tumor cells, compared with IL-2; to explore the therapeutic effect of CTL induction and cytokine gene modification Relationship between the efficacy of tumor cell preparation. METHODS: ①MTT-2 cells were transplanted into C_3H / He and Swiss nu / nu mice. The bladder was anesthetized with pentobarbital and the bladder was exposed. MBT-2 cells and 50μl PBS were injected into the bladder wall. ② retroviral vector design and secretion of cytokines MBT-2 cell line preparation: retroviral vector from Moloney murine leukemia virus. The cytokine cDNA is fused to the promoter in the vector. GM-CSF, IL-1α, IL-1β and IFN-γcDNA were cloned into PBC140, DC /