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作者从多房棘球绦虫(Em)(从感染EM的Meriones unguiculatus中分离得到)中抽提mRNA,反转录成cDNA后,连接到λZAPXR上,构建了含有7×10~6重组子的cDNA基因文库,并以细粒棘球蚴(E.g)谷
The authors extracted mRNA from Echinococcus multilocularis (Em) (isolated from Meriones unguiculatus infected with EM), reverse transcribed into cDNA, and ligated into λZAPXR to construct a cDNA containing 7 × 10 6 recombinants Gene library, and to Echinococcus granulosus (Eg) Valley