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目的：观察复方葶苈注射液对体外新生小牛肺主动脉内皮细胞单层通透性的影响。方法：新生小牛肺主动脉内皮细胞培养于醋酸纤维膜上，分别测定Ｈａｎｋ′ｓ液１∶１０稀释的复方葶苈注射液作用６０分钟前后同一滤膜上正常内皮单层和组胺处理内皮单层的滤过系数（Ｋｆ）和渗透压反射系数（σ）。结果：经１∶１０的复方葶苈注射液作用后，正常内皮单层〔（１１．８０±０．７５）μｌ·ｍｉｎ－１·ｃｍ－２·ｋＰａ－１比（７．７７±０．６７）μｌ·ｍｉｎ－１·ｃｍ－２·ｋＰａ－１〕和组胺处理内皮单层〔（１６．８７±１．１１）μｌ·ｍｉｎ－１·ｃｍ－２·ｋＰａ－１比（１２．８２±１．１９）μｌ·ｍｉｎ－１·ｃｍ－２·ｋＰａ－１〕的Ｋｆ均明显降低（Ｐ均＜０．０１），σ（正常内皮单层σ为０．３２±０．０６比０．４４±０．０３，组胺处理内皮单层σ为０．１９±０．０６比０．２８±０．０６）则均明显升高（Ｐ均＜０．０１）。结论：复方葶苈注射液有降低肺主动脉内皮单层通透性的作用，可减轻肺动脉高压引起的肺水肿。
OBJECTIVE: To observe the effect of compound Fuling injection on the permeability of endothelial monolayer of newborn calf lung aorta in vitro. METHODS: Neonatal calf pulmonary aorta endothelial cells were cultured on acetate membranes. The normal endothelial monolayer and histamine treatment of endothelium on the same filter were performed before and after 60 minutes of compound yttrium injection in Hank’s solution diluted 1:10. Single layer filtration factor (Kf) and osmotic pressure reflection coefficient (σ). RESULTS: After being treated with Compound Radix Injection for 1:10, the normal endothelial monolayer was ((11.80±0.75)μl·min-1·cm-2·kPa-1 ratio (7.77±0. 67) μl · min-1 · cm-2 · kPa-1〕 and histamine treatment of endothelial monolayer [(16.87 ± 1.11) μl · min-1 · cm-2 · kPa-1 ratio (12. The Kf of 82±1.19μl·min-1·cm-2·kPa-1 were all significantly decreased (P<0.01), and σ (normal endothelial monolayer σ was 0.32±0.06). At 0.44 ± 0.03, histamine-treated endothelial monolayer σ was 0.19 ± 0.06 vs. 0.28 ± 0.06. Both were significantly elevated (P <0.01). Conclusion: Compound Fuling injection can reduce the permeability of pulmonary aorta endothelial monolayer, and can reduce pulmonary edema caused by pulmonary hypertension.