论文部分内容阅读
[目的]通过观察纳米碳酸钙亚慢性染毒对大鼠自发活动和学习记忆能力的影响,初步探讨纳米碳酸钙的神经毒性。[方法]选取50只健康雄性SD大鼠,随机分为溶剂对照组、微米碳酸钙组(200 mg/kg)和纳米碳酸钙12.5、50、200 mg/kg组5组,每组10只。滴鼻法每天染毒1次,每周5 d,间歇2 d,持续12周;采用旷场试验和Morris水迷宫试验测试各组大鼠自发活动和学习记忆功能。[结果]微米碳酸钙和纳米碳酸钙组大鼠精神萎靡,但各组间大鼠体质量和脑体比差异均无统计学意义(P>0.05)。旷场试验,各组大鼠在暗箱中中央活动时间、中央运动总路程、站立次数差异均无统计学意义(F值分别为0.799、0.623和0.251,P值均大于0.05)。Morris水迷宫试验,各组大鼠平均潜伏期、平均总路程、穿越平台次数、平台象限滞留时间差异均无统计学意义(F值分别为0.475、0.345、0.046和0.252,P值均大于0.05)。[结论]在本实验剂量条件下,纳米碳酸钙对大鼠自发活动和学习记忆能力无明显影响。
[Objective] To investigate the neurotoxicity of nano-CaCO3 by observing the effects of sub-chronic nano-CaCO3 exposure on spontaneous activity and learning and memory in rats. [Method] Fifty healthy male Sprague-Dawley rats were randomly divided into solvent control group, micronized calcium carbonate group (200 mg / kg), and nano-calcium carbonate group 12.5, 50,200 mg / kg. Dripping nose method once a day, 5 d week, intermittent 2 d for 12 weeks; open field test and Morris water maze test to test spontaneous activity and learning and memory of rats in each group. [Result] The rats in micronized calcium carbonate and calcium carbonate group were apathetic, but there was no significant difference in the body weight and body mass ratio among the three groups (P> 0.05). In the open-field test, there was no significant difference in the central activity time, the total length of central movement and the number of standing between the rats in each group (F = 0.799, 0.623 and 0.251 respectively, P> 0.05). Morris water maze test showed that there was no significant difference in mean incubation period, average total distance, number of crossing platform and residence time of platform quadrats in each group (F value was 0.475,0.345,0.046 and 0.252, P values were greater than 0.05). [Conclusion] Under the experimental conditions, nano-CaCO3 had no significant effect on spontaneous activity and learning and memory ability in rats.