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目的:掌握合川片区艾滋病病毒(HIV)抗体检测,确认实验阳性结果的准确性,以便提高检测质量。方法:按《全国艾滋病检测技术规范》要求进行操作。酶联免疫吸附实验(ELISA)筛查HIV抗体和蛋白免疫印迹试验(WB)确认结果的一致性。结果:2011-2014年共采集1918人份疑似艾滋病血清标本,经采用两种ELISA筛查试剂阳性或一阴一阳的标本经WB确认,阳性1803人份,阳性率为94.0%(1803/1918),不确定为61人份.占被检测标本的3.18%(61/1918),阴性69人份,占被检测标本的3.6%(69/1918)。结论:艾滋病初筛实验结果不完全代表检测样本真实性,必须以确认检测实验报告为准。
Objective: To master the detection of HIV antibody in Hechuan area and confirm the accuracy of the positive results in order to improve the quality of detection. Methods: According to “National AIDS Testing Technical Specifications” requirements for operation. Enzyme-linked immunosorbent assay (ELISA) Screening HIV antibody and Western blotting (WB) to confirm the consistency of the results. Results: A total of 1918 HIV-positive serum samples were collected from 2011 to 2014. The samples with two ELISA screening reagents or one yin-yang were confirmed by WB with 1803 positive samples, the positive rate was 94.0% (1803/1918 ), And the number of uncertainties is 61, accounting for 3.18% (61/1918) of the tested samples and 69 negative samples, accounting for 3.6% (69/1918) of the tested samples. Conclusion: The results of AIDS screening test do not completely represent the authenticity of the test samples, and the test report of confirmation test must prevail.