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目的:通过建立T淋巴细胞(T细胞)免疫功能缺陷的小鼠动物模型,观察壮尔颗粒对小鼠体内细胞因子平衡状态的影响。方法:将48只小鼠随机分为空白组,模型组,玉屏风颗粒组,壮尔颗粒高、中、低剂量组,空白组和模型组ig给予生理盐水,其余各组分别ig给予按玉屏风颗粒溶液(3.38 g·kg-1),壮尔颗粒高、中、低剂量组(4.04,2.02,1.01 g·kg-1)。给药10 d后,空白组经鼻滴入10μL无菌生理盐水,其余各组经鼻滴入10μL肺炎链球菌悬液(密度为1×105CFU/mL),制备小鼠T细胞免疫功能缺陷的动物模型。应用流式微球技术(CBA)分别测定小鼠血清和小鼠脾脏白介素(IL)-2等细胞因子的水平,免疫组织化学染色法检测小鼠脾脏中IL-2和IL-4的表达。结果:与正常组比较,模型组小鼠血清和脾脏中IL-2,肿瘤坏死因子(TNF),干扰素-γ(IFN-γ)的水平均有显著性下降(P<0.05),同时IL-4,IL-6,IL-10,IL-17A的水平有显著性升高(P<0.05)。与模型组比较,壮尔颗粒高、中剂量组可显著提高小鼠血清和脾脏中IL-2,TNF,IFN-γ水平(P<0.05),同时IL-4,IL-6,IL-10,IL-17A的水平均有显著性下降(P<0.05),并且小鼠脾脏IL-2表达上调和IL-4表达下调,有统计学意义(P<0.05)。结论:壮尔颗粒能够通过调节小鼠体内细胞因子的平衡来增强机体免疫功能。
OBJECTIVE: To establish an animal model of immune deficiency in T lymphocytes (T cells) and observe the effect of Cang Er particles on the balance of cytokines in mice. Methods: Forty-eight mice were randomly divided into blank group, model group, Yupingfeng granule group, Zhuanger granule high, medium and low dose groups, blank group and model group ig given normal saline, and the other groups were given ig Screen powder solution (3.38 g · kg-1), Zhuoer granule high, medium and low dose groups (4.04,2.02,1.01 g · kg-1). After 10 d of administration, 10 μL of sterile saline was dripped into the blank group and 10 μL of Streptococcus pneumoniae suspension (density: 1 × 10 5 CFU / mL) Animal model The levels of interleukin-2 (IL-2) and other cytokines in serum and mouse spleen were measured by flow cytometry (CBA). The expression of IL-2 and IL-4 in spleen of mice was detected by immunohistochemical staining. Results: Compared with normal group, the levels of IL-2, TNF and IFN-γ in serum and spleen of model group were significantly decreased (P <0.05) -4, IL-6, IL-10, IL-17A levels were significantly increased (P <0.05). The levels of IL-2, TNF and IFN-γ in Serum and spleen of mice were significantly higher than that of the model group (P <0.05), while the concentrations of IL-4, IL-6 and IL-10 , IL-17A levels were significantly decreased (P <0.05), and the mouse spleen IL-2 expression and IL-4 down regulation, with statistical significance (P <0.05). Conclusion: Weirier granule can enhance immune function by regulating the balance of cytokines in mice.