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乳清蛋白是马乳中主要的蛋白质,不同物种间蛋白质组成存在差异。该研究利用反相高效液相色谱(RP-HPLC)对马乳清中的蛋白进行分离和定量分析,利用基质辅助激光解吸电离飞行时间质谱(MALDI-TOFMS)对主要分离组分进行鉴定。结果表明,马乳乳清蛋白中主要组分为LYS,α-lg和β-lg。利用建立的色谱分析方法对乳清蛋白定量测定结果显示,LYS,α-lg和β-lg在(0.1~1.0)(R~2=0.999 3)、(0.2~0.8)(R~2=0.996 9)和(0.2~1.0)g/L(R~2=0.990 3)线性关系良好。该方法的稳定性、精密度、重现性的相对标准偏差(RSD)均小于5%,加标回收率分别为:88.05%~96.26%,86.03%~95.70%和91.79%~100.97%,能够满足实际检测的需要。测得马乳乳清中的LYS,α-lg和β-lg,含量为别为(3.38±0.32)、(2.64±0.37)及(0.52±0.06)g/L。
Whey protein is the main protein in horse milk, there are differences in protein composition among different species. In this study, RP-HPLC was used to separate and quantify the protein in the whey, and the main components were identified by matrix-assisted laser desorption / ionization time-of-flight mass spectrometry (MALDI-TOFMS). The results showed that the main components of the horse milk whey protein LYS, α-lg and β-lg. The results of quantitative determination of whey protein by using the established chromatographic analysis showed that LYS, α-lg and β-lg showed significant differences between (0.1-1.0) (R 2 = 0.999 3), (0.2-0.8) 9) and (0.2 ~ 1.0) g / L (R ~ 2 = 0.990 3). The relative standard deviations (RSDs) of the stability, precision and reproducibility of the method were all less than 5%. The recovery rates of the methods were 88.05% ~ 96.26%, 86.03% ~ 95.70% and 91.79% ~ 100.97% Meet the actual testing needs. The contents of LYS, α-lg and β-lg in the milk of whey were respectively (3.38 ± 0.32), (2.64 ± 0.37) and (0.52 ± 0.06) g / L.